Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 57598..58091 Replicon plasmid pMCR_J9_7
Accession NZ_CP075067
Organism Escherichia coli strain EC_J_9

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag H4F96_RS25585 Protein ID WP_001230709.1
Coordinates 57598..57852 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag H4F96_RS25590 Protein ID WP_000151456.1
Coordinates 57849..58091 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F96_RS25540 (H4F96_25550) 52628..52789 + 162 WP_001128311.1 hypothetical protein -
H4F96_RS25925 53115..53267 + 153 Protein_60 DUF5431 family protein -
H4F96_RS25545 (H4F96_25555) 53212..53334 + 123 WP_223685802.1 Hok/Gef family protein -
H4F96_RS25550 (H4F96_25560) 53484..54080 + 597 WP_000125165.1 ProQ/FINO family protein -
H4F96_RS25555 (H4F96_25565) 54098..54382 - 285 WP_001162371.1 hypothetical protein -
H4F96_RS25560 (H4F96_25570) 54456..55121 - 666 WP_000532149.1 division plane positioning ATPase MipZ -
H4F96_RS25565 (H4F96_25575) 55418..55594 + 177 WP_249735946.1 hypothetical protein -
H4F96_RS25570 (H4F96_25580) 55710..56039 + 330 WP_001542022.1 hypothetical protein -
H4F96_RS25575 (H4F96_25585) 56440..56955 + 516 WP_001025393.1 J domain-containing protein -
H4F96_RS25580 (H4F96_25590) 57059..57601 + 543 WP_001083904.1 hypothetical protein -
H4F96_RS25585 (H4F96_25595) 57598..57852 + 255 WP_001230709.1 hypothetical protein Toxin
H4F96_RS25590 (H4F96_25600) 57849..58091 + 243 WP_000151456.1 hypothetical protein Antitoxin
H4F96_RS25595 (H4F96_25605) 58364..58546 + 183 WP_071599349.1 hypothetical protein -
H4F96_RS25600 (H4F96_25610) 58662..58955 + 294 WP_000673428.1 hypothetical protein -
H4F96_RS25605 (H4F96_25615) 59444..59692 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -
H4F96_RS25610 (H4F96_25620) 59689..60036 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin -
H4F96_RS25930 60124..60567 - 444 WP_023223351.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..60959 60959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(6-129)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T202799 WP_001230709.1 NZ_CP075067:57598-57852 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T202799 NZ_CP099623:4717736-4717918 [Paraburkholderia fungorum]
ATGAACAGCGCCGAAGTCGTCAAAGTCATTCAAGCGGATGGCTGGCGGTTGATCCGGATATCGGGCAGTCACCATCATTT
TCGGCACGCGGTAAAAGCCGGTCTCGTGACCATCCCGCATCCGAAAAAAGATCTTCCGCCCGGCACATTGAACAGCATCC
TGAAACAGGCGGGTCTGAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT202799 WP_000151456.1 NZ_CP075067:57849-58091 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT202799 NZ_CP099623:4717915-4718325 [Paraburkholderia fungorum]
ATGAAAAATCTGATTTTCCCGATCGCAATCGAACCTGGTGACACGCACCACGCGTTCGGCGTGATCGTCCCCGATATTCC
GGGTTGTCATTCGGCGGGTAGTTCGTTGGAAGAAGCCTACGCGAACGCAAAAGAAGCAATCGAGGCGCATCTGGACACGC
TGCTCGATGAAGGCCTGCCGATTCCCGAGCGGCTAACACTGGAGGAACATCGGCGCAATCCCGACTACGCCGGTTTCACC
TGGGGATTTGTCACCACGCGCAATATTCCGGCGCTGAAGAAAGCCGTGCGCATCAATATCTCGCTGCCCGAGGCGCTGGT
TCACGATATCGACGCTTACGCCCAGGCACGCGGCATGTCGCGCTCGGCGTTTCTCGCGCTGGCCGCCGAGCACGAGATGG
CGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References