Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 862236..862890 Replicon chromosome
Accession NZ_CP074680
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KIM92_RS04195 Protein ID WP_000244772.1
Coordinates 862483..862890 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KIM92_RS04190 Protein ID WP_000354046.1
Coordinates 862236..862502 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS04165 (857524) 857524..858267 + 744 WP_000951964.1 SDR family oxidoreductase -
KIM92_RS04170 (858324) 858324..859757 - 1434 WP_166739776.1 6-phospho-beta-glucosidase BglA -
KIM92_RS04175 (859802) 859802..860113 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
KIM92_RS04180 (860277) 860277..860936 + 660 WP_000250274.1 hemolysin III family protein -
KIM92_RS04185 (861013) 861013..861993 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KIM92_RS04190 (862236) 862236..862502 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KIM92_RS04195 (862483) 862483..862890 + 408 WP_000244772.1 protein YgfX Toxin
KIM92_RS04200 (862930) 862930..863451 - 522 WP_001055874.1 flavodoxin FldB -
KIM92_RS04205 (863563) 863563..864459 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KIM92_RS04210 (864484) 864484..865194 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KIM92_RS04215 (865200) 865200..866933 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T202621 WP_000244772.1 NZ_CP074680:862483-862890 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T202621 NZ_CP099531:c4477258-4477064 [Xanthomonas sacchari]
ATGCCCCCGAAAATCCGCGATGTGCTGCAACAACTCAAGGACGCAGGCTGGCAGCAAGTCGGCCAGACCGGCAGCCACCG
CCAGTTCAAGCATCCTGTCTACCCAGGCCGCGTGACAGTGGCCGGCAAGCCGAGCGATGATGTGGCTCCCGGCACCCTGA
ACAGCATTCTCAAACAGGCCAGCCTGAAGAGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT202621 WP_000354046.1 NZ_CP074680:862236-862502 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT202621 NZ_CP099531:c4477058-4476846 [Xanthomonas sacchari]
ATGCGCTACGCCGTTGTCATCGAGCAAGCGGGCAACAACTACTCCGCGTACGTGCCGGATCTGCCGGGGTGTGTGGCGAC
GGGTGCGACGACTGCGGAGGTGGAGGGATTGATCCGCGAGGCGATTAAGCTGCATCTGGCTGGCATGCGCGAGGATGGGG
AAGTGATTCCGCCGCCGTCGAGTCAGGTTGAGTATGTGGATGTGGCGGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References