Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 353798..354444 Replicon chromosome
Accession NZ_CP074677
Organism Klebsiella pneumoniae strain MEI009

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag KIM99_RS01635 Protein ID WP_004174016.1
Coordinates 353798..354145 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1F2LZT5
Locus tag KIM99_RS01640 Protein ID WP_008806992.1
Coordinates 354145..354444 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM99_RS01625 (349724) 349724..351157 + 1434 WP_002920564.1 glycogen synthase GlgA -
KIM99_RS01630 (351175) 351175..353622 + 2448 WP_002920561.1 glycogen phosphorylase -
KIM99_RS01635 (353798) 353798..354145 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KIM99_RS01640 (354145) 354145..354444 + 300 WP_008806992.1 XRE family transcriptional regulator Antitoxin
KIM99_RS01645 (354507) 354507..356015 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
KIM99_RS01650 (356220) 356220..356549 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
KIM99_RS01655 (356600) 356600..357430 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
KIM99_RS01660 (357480) 357480..358238 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T202595 WP_004174016.1 NZ_CP074677:353798-354145 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T202595 NZ_CP099513:c4776573-4776163 [Klebsiella sp. ZYC-1]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCATGGCGCGCGCAGTGGTTTTCACTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTAGTGTTCGACTGCG
TACGCAGCCAGCGGCGGATCCACGCCCGTCAGGGCGAAATAAAGCTGCTGATCGATTCGCGTCTGCGCTGGCAAAAGGCG
GAGTGGGAGATTATCGGTACGCCGTGGGTAATCAACAGCGGCATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGTAC
CCAGCATTTATGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGCGACCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10814.61 Da        Isoelectric Point: 10.1464

>AT202595 WP_008806992.1 NZ_CP074677:354145-354444 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT202595 NZ_CP099513:c4776820-4776554 [Klebsiella sp. ZYC-1]
ATGGACATTAACAATAAAGCCCGTATCCATTGGGCATGTCGTCGAGGTATGCGTGAACTCGATATATCCATCATGCCGTT
TTTCGAGCATGAGTACGATACGCTAAGCGATGGCGATAAGGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TCTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCTGAACTGCAGCGGATGGTAAAATTAATTCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZT5

References