Detailed information of TA system TA202459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 309233..310821
• Replicon: chromosome
• Accession: NZ_CP074580
• Organism: Klebsiella pneumoniae strain B30155

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9J6RYF8
• Locus tag: KH752_RS01535
• Protein ID: WP_004892016.1
• Coordinates: 309233..310555 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KH752_RS01540
• Protein ID: WP_004176198.1
• Coordinates: 310555..310821 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH752_RS01520 (KH752_01520)	304289..306790	+	2502	WP_004892009.1	fimbria/pilus outer membrane usher protein	-
KH752_RS01525 (KH752_01525)	306778..307782	+	1005	WP_004892012.1	fimbrial protein	-
KH752_RS01530 (KH752_01530)	307924..309216	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
KH752_RS01535 (KH752_01535)	309233..310555	-	1323	WP_004892016.1	type II toxin-antitoxin system HipA family toxin	Toxin
KH752_RS01540 (KH752_01540)	310555..310821	-	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KH752_RS01545 (KH752_01545)	311086..311412	-	327	WP_004892019.1	hydroxyisourate hydrolase	-
KH752_RS01550 (KH752_01550)	311409..311909	-	501	WP_004892023.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
KH752_RS01555 (KH752_01555)	311887..313266	-	1380	WP_004176204.1	nucleobase:cation symporter-2 family protein	-
KH752_RS01560 (KH752_01560)	313611..314765	+	1155	WP_004892025.1	FAD-dependent urate hydroxylase HpxO	-
KH752_RS01565 (KH752_01565)	314746..315669	-	924	WP_004892028.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	120590..346303	225713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49141.44 Da
• Isoelectric Point: 7.5357

>T202459 WP_004892016.1 NZ_CP074580:c310555-309233 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T202459 NZ_CP099401:191661-192062 [Rhizobium sp. Pop5]
ATGCCGCTTTATATGCTCGACACCAATATGTGCATCTACCTGATGAAGGAGCAGCCGCCGCAGGTCGCACGCCGTTTTGC
TCAATGCCGTGTCGGCGACGTCGTCATGTCGGCCATTACATTTGCGGAACTTGAATTTGGAGTCTTCGCCTCGATGAACG
CGGAAAAGGAGCGGGCAAACCTGGAATTCCTTATAGAAGCGATCCCAGTTGCGCCCTTCGATGCCAATGCGGGGCGGTCC
TATGGGCCAGTCAGATTTGCCACGCGCGAACGGAAAAAAGATCATCTGGACAAATTGATAGCCGCTCATGCGCTCTCGAT
TGACGCGATCCTTGTGACCAACAATGTGCGGGACTTTTCCGTCTACGAACGGTTGCGGATCGAGAACTGGTTGTTGTCGT
GA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT202459 WP_004176198.1 NZ_CP074580:c310821-310555 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT202459 NZ_CP099401:191425-191661 [Rhizobium sp. Pop5]
ATGCACACGACGAAGGTATTCAAAAACGGCAATTCGCAGGCAGTGCGCATTCCCGCGGATCTGGCTTACAGTCAGACCGA
GATGGATCTTGAAATCGAGCGGATTGGTGACGAGCTTCGAATCCGCCCGCTGAAACCATCCCTGGAAGGCGTGCTGTCGC
GTTTCGCAAGGTTTGACCGCTCGTTCATGGCGGAGGGACGCGGCGATCAGGAGCAGGCCGAGCGGGATACGCTTTGA