Detailed information of TA system TA201998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 676053..676701
• Replicon: chromosome
• Accession: NZ_CP074373
• Organism: Virgibacillus sp. NKC19-16

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KFZ58_RS03630
• Protein ID: WP_235793497.1
• Coordinates: 676342..676701 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KFZ58_RS03625
• Protein ID: WP_235793496.1
• Coordinates: 676053..676337 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFZ58_RS03605 (KFZ58_03610)	671139..671498	+	360	WP_235794649.1	holo-ACP synthase	-
KFZ58_RS03610 (KFZ58_03615)	671556..673082	+	1527	WP_235793493.1	NAD(P)H-hydrate dehydratase	-
KFZ58_RS03615 (KFZ58_03620)	673134..674165	+	1032	WP_235793494.1	outer membrane lipoprotein carrier protein LolA	-
KFZ58_RS03620 (KFZ58_03625)	674797..675924	+	1128	WP_235793495.1	alanine racemase	-
KFZ58_RS03625 (KFZ58_03630)	676053..676337	+	285	WP_235793496.1	antitoxin	Antitoxin
KFZ58_RS03630 (KFZ58_03635)	676342..676701	+	360	WP_235793497.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KFZ58_RS03635 (KFZ58_03640)	676902..677771	+	870	WP_235793498.1	RsbT co-antagonist protein RsbRA	-
KFZ58_RS03640 (KFZ58_03645)	677768..678133	+	366	WP_235793499.1	STAS domain-containing protein	-
KFZ58_RS03645 (KFZ58_03650)	678136..678537	+	402	WP_235793500.1	anti-sigma regulatory factor	-
KFZ58_RS03650 (KFZ58_03655)	678638..679651	+	1014	WP_235793501.1	PP2C family protein-serine/threonine phosphatase	-
KFZ58_RS03655 (KFZ58_03660)	679757..680086	+	330	WP_235793502.1	STAS domain-containing protein	-
KFZ58_RS03660 (KFZ58_03665)	680086..680565	+	480	WP_235793503.1	anti-sigma B factor RsbW	-
KFZ58_RS03665 (KFZ58_03670)	680537..681328	+	792	WP_235793504.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13377.58 Da
• Isoelectric Point: 9.4870

>T201998 WP_235793497.1 NZ_CP074373:676342-676701 [Virgibacillus sp. NKC19-16]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDNEMMKKIDQALEISLGLKDMYGH

Nucleotide

• Length: 360 bp

>T201998 NZ_CP099254:c67277-66957 [Citrobacter freundii]
ATGCATATTTCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGATACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCGGGAGTGAATCTTACTGATGCTGTTAATTTCCTTGTGGAACGATTCGATCTTGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACACTGGATAACATCTTTGGATGTTCATCGGGCACAGTTCAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10829.11 Da
• Isoelectric Point: 4.2608

>AT201998 WP_235793496.1 NZ_CP074373:676053-676337 [Virgibacillus sp. NKC19-16]
MSESLQEVLVKLPTNLLNEVDGLMKYENSDLSDFISQATKNYLDQKRDEHIQQFRETMKKGYQEMGRINLTIASEAFQAE
EEAENTLERSVIGV

Nucleotide

• Length: 285 bp

>AT201998 NZ_CP099254:c67676-67311 [Citrobacter freundii]
ATGCAATCGACCACCTTATCCGGCTCATCAACGGAGAACGCATCCTGCCTTCAGTGGGGATTAAAACGTAATATCAAGCC
GTGCTTCGGCGCTCGCCTGGTACAGGAAGGCAACCGTCTCTACTTTCTGACTGATCGGGCTGGGTTTGATGGCTCATTCA
GTGACGATGAAGCTTTGCGTCTGGACCAGGCATTTCCACTGATGATGAAACAGCTTGAGCGGATGCTCACGACTGGCGAG
CTTGATCCTCGCAGCCAGCACTGCGTCACAAGGCATCACAACGGACTTACTTGTGAAGCTGACACTCTCGGCTCTCATGG
CTACGTTTACATTGCCATATATCCCCAGTCAGCTCTAACCAGATAA