Detailed information of TA system TA201991

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59803..60057
• Replicon: plasmid unnamed2
• Accession: NZ_CP074368
• Organism: Escherichia coli strain ZYB62

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KG092_RS24830
• Protein ID: WP_001312851.1
• Coordinates: 59803..59952 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	59996..60057 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KG092_RS24800 (54930)	54930..55748	+	819	WP_024199078.1	anaerobic sulfite reductase subunit AsrB	-
KG092_RS24805 (55759)	55759..56772	+	1014	WP_000109841.1	sulfite reductase subunit C	-
KG092_RS24810 (57629)	57629..58486	-	858	WP_000774302.1	incFII family plasmid replication initiator RepA	-
KG092_RS24815 (58479)	58479..58961	-	483	WP_001443034.1	hypothetical protein	-
KG092_RS24820 (58954)	58954..59028	-	75	WP_001442103.1	RepA leader peptide Tap	-
KG092_RS24825 (59262)	59262..59519	-	258	WP_000083834.1	replication regulatory protein RepA	-
KG092_RS24830 (59803)	59803..59952	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (59996)	59996..60057	+	62	NuclAT_0	-	Antitoxin
- (59996)	59996..60057	+	62	NuclAT_0	-	Antitoxin
- (59996)	59996..60057	+	62	NuclAT_0	-	Antitoxin
- (59996)	59996..60057	+	62	NuclAT_0	-	Antitoxin
KG092_RS24835 (60353)	60353..60664	+	312	WP_000802277.1	hypothetical protein	-
KG092_RS24840 (60845)	60845..60931	-	87	Protein_54	endonuclease	-
KG092_RS24845 (61129)	61129..61317	-	189	WP_001490801.1	hypothetical protein	-
KG092_RS24850 (61452)	61452..62012	-	561	WP_000139310.1	fertility inhibition protein FinO	-
KG092_RS24855 (62067)	62067..62813	-	747	WP_000205776.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..87829	87829

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T201991 WP_001312851.1 NZ_CP074368:c59952-59803 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T201991 NZ_CP099252:2600739-2601224 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCTGCACCGCTATCTTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCTCAGGGCGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGAGAGCC
ATCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGAACCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTATCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTTCGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACGATTCTATTTACACCACGGATTCAAAGCCTCCACCACCCAGGAACGCACACTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT201991 NZ_CP074368:59996-60057 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin