Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5172472..5173025 Replicon chromosome
Accession NZ_CP074182
Organism Raoultella planticola strain JBIWA001

Toxin (Protein)


Gene name ccdB Uniprot ID A0A443VE20
Locus tag KGP19_RS24550 Protein ID WP_057108625.1
Coordinates 5172472..5172786 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A443VE25
Locus tag KGP19_RS24555 Protein ID WP_057108464.1
Coordinates 5172789..5173025 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP19_RS24520 (KGP19_24455) 5168099..5168497 + 399 WP_057108599.1 cag pathogenicity island Cag12 family protein -
KGP19_RS24525 (KGP19_24460) 5168546..5168848 + 303 WP_057108600.1 TrbM/KikA/MpfK family conjugal transfer protein -
KGP19_RS24530 (KGP19_24465) 5168897..5169202 + 306 WP_072146387.1 dpoa decarboxylase -
KGP19_RS24535 (KGP19_24470) 5169274..5169627 + 354 WP_196239630.1 hypothetical protein -
KGP19_RS24540 (KGP19_24475) 5169978..5171714 + 1737 WP_057108601.1 type IV secretory system conjugative DNA transfer family protein -
KGP19_RS24545 (KGP19_24480) 5171723..5172475 + 753 WP_064388594.1 MobC family replication-relaxation protein -
KGP19_RS24550 (KGP19_24485) 5172472..5172786 - 315 WP_057108625.1 CcdB family protein Toxin
KGP19_RS24555 (KGP19_24490) 5172789..5173025 - 237 WP_057108464.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KGP19_RS24560 (KGP19_24495) 5173348..5174292 - 945 WP_057108463.1 zincin-like metallopeptidase domain-containing protein -
KGP19_RS24565 (KGP19_24500) 5175170..5175463 + 294 WP_223526217.1 LysR substrate-binding domain-containing protein -
KGP19_RS24570 (KGP19_24505) 5175396..5175614 + 219 Protein_4820 transposase -
KGP19_RS24575 5176247..5176543 + 297 WP_223525992.1 hypothetical protein -
KGP19_RS24580 (KGP19_24515) 5176686..5176865 + 180 Protein_4822 transcriptional regulator -
KGP19_RS24585 (KGP19_24520) 5176946..5177270 + 325 Protein_4823 helix-turn-helix domain-containing protein -
KGP19_RS24590 (KGP19_24525) 5177303..5177617 - 315 Protein_4824 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 5153135..5196369 43234


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(10-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11487.35 Da        Isoelectric Point: 7.9888

>T201795 WP_057108625.1 NZ_CP074182:c5172786-5172472 [Raoultella planticola]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVDRYPAGRRPDRLVPVVRLTDGQEYAVMTHELASVPVRALG
AVFCDASQYRTQVKAAIDFLIDGI

Download         Length: 315 bp

>T201795 NZ_CP098769:741898-742383 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9012.08 Da        Isoelectric Point: 6.2206

>AT201795 WP_057108464.1 NZ_CP074182:c5173025-5172789 [Raoultella planticola]
MTAKQRNTLSVTMTVERALLVRAREAGINLSATLTAALDAELRHHEAKKWQEENREAIDALNRFHDEYGCFSDEYRTF

Download         Length: 237 bp

>AT201795 NZ_CP098769:741608-741901 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443VE20

Antitoxin

Source ID Structure
AlphaFold DB A0A443VE25

References