Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3604093..3604713 Replicon chromosome
Accession NZ_CP074154
Organism Enterobacter asburiae strain JBIWA007

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag KGP18_RS17460 Protein ID WP_008499287.1
Coordinates 3604495..3604713 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag KGP18_RS17455 Protein ID WP_008499288.1
Coordinates 3604093..3604467 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGP18_RS17445 (KGP18_17420) 3599219..3600412 + 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KGP18_RS17450 (KGP18_17425) 3600435..3603581 + 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit AcrB -
KGP18_RS17455 (KGP18_17430) 3604093..3604467 + 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
KGP18_RS17460 (KGP18_17435) 3604495..3604713 + 219 WP_008499287.1 HHA domain-containing protein Toxin
KGP18_RS17465 (KGP18_17440) 3604919..3605470 + 552 WP_223534847.1 maltose O-acetyltransferase -
KGP18_RS17470 (KGP18_17445) 3605588..3606055 + 468 WP_023310598.1 YlaC family protein -
KGP18_RS17475 (KGP18_17450) 3606027..3607487 - 1461 WP_223534848.1 PLP-dependent aminotransferase family protein -
KGP18_RS17480 (KGP18_17455) 3607589..3608299 + 711 WP_126796955.1 GNAT family protein -
KGP18_RS17485 (KGP18_17460) 3608296..3608436 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KGP18_RS17490 (KGP18_17465) 3608439..3608699 - 261 WP_010428154.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T201691 WP_008499287.1 NZ_CP074154:3604495-3604713 [Enterobacter asburiae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T201691 NZ_CP098719:607987-608277 [Yersinia ruckeri]
ATGACCTTTAATATCGACTTTGACGAACGGGCATTAAAAGAATGGCACAAACTAGATAAAACTATCCGAGAGCAATTTAA
AAAGAAACTAAGAAAATTACAGGAAAATCCCTATATTGAGTCAGCACGTTTGCACGGTGATCTGGCTGGTTGTTTCAAAA
TAAAATTACGCGCATCTGGCTTTCGGTTGATTTACCAAGTTATCGATGAGGAGATTGTGATTTTGATCGTTGCTGTTGGT
AAACGGGAAGATGAAAAAGCTTATGATCTTGCCAAGAAGCGAATCCAATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT201691 WP_008499288.1 NZ_CP074154:3604093-3604467 [Enterobacter asburiae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT201691 NZ_CP098719:607743-607997 [Yersinia ruckeri]
ATGAGTACTATCCAAATTCGTGTGGATGATGAACTGAAGAAAAGTGCATATCAGGCGTTTGAAAAACTGAATCTTTCGCC
TTCTGATGCACTACGCCTGTTCCTACGTTATGTTGCAGAGAACGAAAAGCTGCCTTTTTCAGAAGTATCCGTCATGGTCA
GTAATCAAGATGAGGATGCTGATATTTTGGCTGTTGTCCGTGAACGTTTGAAAAATCCTGCAAAGCGTATCAGAGTAAAT
ATTGATGACCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References