Detailed information of TA system TA201599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 6049669..6050321
• Replicon: chromosome
• Accession: NZ_CP074140
• Organism: Serratia sp. JSRIV004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U2LU32
• Locus tag: KGP21_RS28775
• Protein ID: WP_021181419.1
• Coordinates: 6049980..6050321 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KGP21_RS28770
• Protein ID: WP_021181420.1
• Coordinates: 6049669..6049983 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP21_RS28755 (KGP21_28660)	6045423..6047252	+	1830	WP_218520083.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
KGP21_RS28760 (KGP21_28665)	6047375..6048490	+	1116	WP_223536728.1	hypothetical protein	-
KGP21_RS28765 (KGP21_28670)	6048492..6049571	+	1080	WP_223500571.1	GNAT family N-acetyltransferase	-
KGP21_RS28770 (KGP21_28675)	6049669..6049983	-	315	WP_021181420.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
KGP21_RS28775 (KGP21_28680)	6049980..6050321	-	342	WP_021181419.1	hypothetical protein	Toxin
KGP21_RS28780 (KGP21_28685)	6050689..6051729	+	1041	WP_021181417.1	phosphate ABC transporter substrate-binding protein PstS	-
KGP21_RS28785 (KGP21_28690)	6051820..6052776	+	957	WP_024527373.1	phosphate ABC transporter permease PstC	-
KGP21_RS28790 (KGP21_28695)	6052778..6053668	+	891	WP_059199278.1	phosphate ABC transporter permease PstA	-
KGP21_RS28795 (KGP21_28700)	6053716..6054492	+	777	WP_021181414.1	phosphate ABC transporter ATP-binding protein PstB	-
KGP21_RS28800 (KGP21_28705)	6054505..6055236	+	732	WP_024484696.1	phosphate signaling complex protein PhoU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Antitoxin MqsA/HigA-2 (29-99)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13473.66 Da
• Isoelectric Point: 9.7468

>T201599 WP_021181419.1 NZ_CP074140:c6050321-6049980 [Serratia sp. JSRIV004]
MDAIFVELSIFEAKRADYLSDDEFKTFQQMLLINPFCGDVIQHTGGLRKVRFADHRRGKGKRGGLRVIYYWWLEKSHFLL
FVIYDKDKKDDLSRNEREMLRRMLEILKQGGTL

Nucleotide

• Length: 342 bp

>T201599 NZ_CP098689:1237936-1238079 [Rickettsia rickettsii]
ATGAGAGATCATGCCCTTATAGGTAATTGGAAAGATTGTAGAGATTGTCATATTAAAGCGGATTTAGTATTAATTTATAG
AAAACCCGATGCTGACACATTGGAGTTAATACAATTAGGTTCAAACAGTACACTTGGTTTTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11870.69 Da
• Isoelectric Point: 7.2018

>AT201599 WP_021181420.1 NZ_CP074140:c6049983-6049669 [Serratia sp. JSRIV004]
MSELSLFEELSQGMKDWSDYNEGKITLKTHAVSLKKNISMSPDELKAIREKLRLSQAVFADYLHTGVTTYQNWEQGRAKP
NKQALLLIRMVEKDPMTLDTLAAL

Nucleotide

• Length: 315 bp

>AT201599 NZ_CP098689:1237581-1237844 [Rickettsia rickettsii]
ATGTCTGCTGATTCTATTGTGCGAGCCCGTATTAATGAAGACGTGAAAGAAGAAGCAGCTTTAGTGCTTGCTGCTATGGG
ACTTACTTTATCTGATGCTGTTCGTATGCTGTTATTTCGTGTTGCTCGTGAAAAAGCATTGCCGTTTGAACCACTTATCC
CAAATGATGAAACAATTAAAGCTATGAAAGCTGCAAGAAGCGGCAAATTAGTTCATGTTGGTAATATCAATAATTTGTTA
TCTGATTTAAATGAGAACGATTAA

Structures

Toxin

• AlphaFold DB: U2LU32