Detailed information of TA system TA201576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 241408..242054
• Replicon: chromosome
• Accession: NZ_CP074140
• Organism: Serratia sp. JSRIV004

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KGP21_RS01050
• Protein ID: WP_021807728.1
• Coordinates: 241408..241758 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A370SDA2
• Locus tag: KGP21_RS01055
• Protein ID: WP_021807729.1
• Coordinates: 241755..242054 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGP21_RS01030 (KGP21_01020)	237267..238286	+	1020	WP_071681699.1	dipeptide ABC transporter permease DppB	-
KGP21_RS01035 (KGP21_01025)	238298..239200	+	903	WP_218520002.1	dipeptide ABC transporter permease DppC	-
KGP21_RS01040 (KGP21_01030)	239215..240195	+	981	WP_021807726.1	dipeptide ABC transporter ATP-binding protein	-
KGP21_RS01045 (KGP21_01035)	240192..241181	+	990	WP_021807727.1	peptide ABC transporter ATP-binding protein	-
KGP21_RS01050 (KGP21_01040)	241408..241758	+	351	WP_021807728.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KGP21_RS01055 (KGP21_01045)	241755..242054	+	300	WP_021807729.1	helix-turn-helix transcriptional regulator	Antitoxin
KGP21_RS01060 (KGP21_01050)	242216..243871	-	1656	WP_094982245.1	cellulose biosynthesis protein BcsG	-
KGP21_RS01065 (KGP21_01055)	243874..244071	-	198	WP_021807731.1	cellulose biosynthesis protein BcsF	-
KGP21_RS01070 (KGP21_01060)	244068..245630	-	1563	WP_094982246.1	cellulose biosynthesis protein BcsE	-
KGP21_RS01075 (KGP21_01065)	245825..246019	+	195	WP_021807733.1	cellulose biosynthesis protein BcsR	-
KGP21_RS01080 (KGP21_01070)	246023..246760	+	738	WP_021807734.1	cellulose biosynthesis protein BcsQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13591.49 Da
• Isoelectric Point: 5.2279

>T201576 WP_021807728.1 NZ_CP074140:241408-241758 [Serratia sp. JSRIV004]
MWTVIFTSTFDSWLQEQEEGMQEKVLADLMNLETYGPQLPRPYADTVKGSRYKNMKELRVQYSGRPIRAFFVFDPKRQAI
VLCAGDKSNDKRFYETMIRIADDEFTAYLATQEVSK

Nucleotide

• Length: 351 bp

>T201576 NZ_CP098548:c1635697-1635515 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGTCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10894.65 Da
• Isoelectric Point: 5.8719

>AT201576 WP_021807729.1 NZ_CP074140:241755-242054 [Serratia sp. JSRIV004]
MKTLRDAVAARSLESQARIKEMADEMMLETGLQLMRDELQLSQKSLAEAMGVSQSAVAQIEQRGNDVKLATLKRYIEAMG
GKLSLTVEMPEGGGRVFHI

Nucleotide

• Length: 300 bp

>AT201576 NZ_CP098548:c1635413-1635012 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Antitoxin

• AlphaFold DB: A0A370SDA2