Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 848394..849192 Replicon chromosome
Accession NZ_CP074052
Organism Escherichia coli strain PD23

Toxin (Protein)


Gene name yeeV Uniprot ID A0A1M0HL60
Locus tag KFU85_RS04010 Protein ID WP_000854692.1
Coordinates 848394..848771 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A1L4NTN5
Locus tag KFU85_RS04015 Protein ID WP_001605875.1
Coordinates 848818..849192 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU85_RS03980 (843528) 843528..844676 - 1149 WP_000905920.1 capsule polysaccharide export inner-membrane protein KpsE -
KFU85_RS03985 (844748) 844748..845731 - 984 WP_001298261.1 KpsF/GutQ family sugar-phosphate isomerase -
KFU85_RS03990 (846542) 846542..846712 - 171 Protein_784 IS110 family transposase -
KFU85_RS03995 (847048) 847048..847893 - 846 WP_032292312.1 DUF4942 domain-containing protein -
KFU85_RS04000 (847975) 847975..848172 - 198 WP_023563519.1 DUF957 domain-containing protein -
KFU85_RS04005 (848248) 848248..848397 - 150 Protein_787 DUF5983 family protein -
KFU85_RS04010 (848394) 848394..848771 - 378 WP_000854692.1 TA system toxin CbtA family protein Toxin
KFU85_RS04015 (848818) 848818..849192 - 375 WP_001605875.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
KFU85_RS04020 (849266) 849266..849487 - 222 WP_000692346.1 DUF987 domain-containing protein -
KFU85_RS04025 (849556) 849556..850032 - 477 WP_001186725.1 RadC family protein -
KFU85_RS04030 (850048) 850048..850533 - 486 WP_000214392.1 antirestriction protein -
KFU85_RS04035 (850625) 850625..851443 - 819 WP_001234717.1 DUF932 domain-containing protein -
KFU85_RS04040 (851561) 851561..851756 - 196 Protein_794 DUF905 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14119.12 Da        Isoelectric Point: 7.3249

>T201309 WP_000854692.1 NZ_CP074052:c848771-848394 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWHTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Download         Length: 378 bp

>T201309 NZ_CP098330:4118461-4118802 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13594.42 Da        Isoelectric Point: 5.4554

>AT201309 WP_001605875.1 NZ_CP074052:c849192-848818 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT201309 NZ_CP098330:4118123-4118440 [Citrobacter freundii]
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0HL60

Antitoxin

Source ID Structure
AlphaFold DB A0A1L4NTN5

References