Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 855530..856184 Replicon chromosome
Accession NZ_CP074047
Organism Escherichia coli strain PD7

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU78_RS04220 Protein ID WP_000244777.1
Coordinates 855777..856184 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU78_RS04215 Protein ID WP_000354046.1
Coordinates 855530..855796 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU78_RS04190 (850699) 850699..851442 + 744 WP_000951964.1 SDR family oxidoreductase -
KFU78_RS04195 (851499) 851499..852932 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KFU78_RS04200 (852977) 852977..853288 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KFU78_RS04205 (853452) 853452..854111 + 660 WP_000250274.1 hemolysin III family protein -
KFU78_RS04210 (854307) 854307..855287 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU78_RS04215 (855530) 855530..855796 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU78_RS04220 (855777) 855777..856184 + 408 WP_000244777.1 protein YgfX Toxin
KFU78_RS04225 (856224) 856224..856745 - 522 WP_001055874.1 flavodoxin FldB -
KFU78_RS04230 (856857) 856857..857753 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU78_RS04235 (857778) 857778..858488 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU78_RS04240 (858494) 858494..860227 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T201286 WP_000244777.1 NZ_CP074047:855777-856184 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201286 NZ_CP098322:c1911132-1910848 [Burkholderia gladioli]
ATGCCCGATTACGCCGAATTCGATGCGGCCGACTATCTCGACAATGACGAAACCGTGGCGGAATTTCTCGCGGTTGCGGC
CGAGGATCCCGATCCCGCAGTGTTCCTTGCTGCCGTGGCCGCTGTCGCACGCGCGCGCGGGATGACCAAGGTGGCGTCGG
AAGCCGGCCTTGGACGGGTCACCTTGCACAAGGCGCTGACGCCTGGCGCTCACCCTCGATATGAGACCGTGCAGAAGATC
CTGCGCTCGCTGGGCCTTCGCATGACCTTCACCGTCGCCCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201286 WP_000354046.1 NZ_CP074047:855530-855796 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201286 NZ_CP098322:c1911432-1911136 [Burkholderia gladioli]
ATGGTAACTTTGCTGCGCTCCAGTGAATTCACAAACTGGCTTTCCAGCCTGCGTGATCAGCGCGGCAAAGCCCGAATCGC
CGCACGCCTGATCAGTGCCCAGCTAGGCAATTTTGGCGAGTACCGCGTGATAGGCGAAGGCGTCCGCGAAATGAAGGTCG
ACTTCGGGCCGGGTTATCGCATCTACTATGTCCGTCGCGCCGAGATCGTCTATGTCCTTCTCTGCGGTAGCGATAAATCT
ACACAGAAAAAAGACATCAAGCGCGCGCTCCAGATGGCGCGCGAACTGAAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References