Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 12975..13497 Replicon plasmid pYLPK13a
Accession NZ_CP074029
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag KFU76_RS23255 Protein ID WP_000220560.1
Coordinates 12975..13256 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag KFU76_RS23260 Protein ID WP_000121743.1
Coordinates 13246..13497 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS23220 (KFU76_23065) 9244..9516 + 273 Protein_11 IS1 family transposase -
KFU76_RS23225 (KFU76_23070) 9536..9733 - 198 WP_000844102.1 hypothetical protein -
KFU76_RS23230 (KFU76_23075) 9832..10800 - 969 WP_000654802.1 IS5-like element IS903B family transposase -
KFU76_RS23235 (KFU76_23080) 10822..11313 - 492 WP_240290727.1 restriction endonuclease -
KFU76_RS23240 (KFU76_23085) 11501..11812 - 312 WP_001452736.1 hypothetical protein -
KFU76_RS23245 (KFU76_23090) 11848..12162 - 315 WP_000734776.1 TrbM/KikA/MpfK family conjugal transfer protein -
KFU76_RS23255 (KFU76_23100) 12975..13256 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KFU76_RS23260 (KFU76_23105) 13246..13497 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
KFU76_RS23265 (KFU76_23110) 14731..15567 + 837 WP_001050931.1 RepB family plasmid replication initiator protein -
KFU76_RS23270 (KFU76_23115) 15607..16053 + 447 Protein_21 hypothetical protein -
KFU76_RS23275 (KFU76_23120) 16190..16546 + 357 WP_000456533.1 DNA distortion polypeptide 3 -
KFU76_RS23280 (KFU76_23125) 16543..17520 - 978 WP_024245156.1 hypothetical protein -
KFU76_RS23285 (KFU76_23130) 17545..17826 - 282 WP_001185482.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-32 / qnrB19 - 1..32666 32666


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T201186 WP_000220560.1 NZ_CP074029:c13256-12975 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T201186 NZ_CP098229:3610702-3611244 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT201186 WP_000121743.1 NZ_CP074029:c13497-13246 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT201186 NZ_CP098229:3610407-3610718 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References