Detailed information of TA system TA201177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2834807..2835409
• Replicon: chromosome
• Accession: NZ_CP074028
• Organism: Escherichia coli strain PK13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KFU76_RS13695
• Protein ID: WP_000897305.1
• Coordinates: 2835098..2835409 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KFU76_RS13690
• Protein ID: WP_000356397.1
• Coordinates: 2834807..2835097 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU76_RS13665 (2830732)	2830732..2831634	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KFU76_RS13670 (2831631)	2831631..2832266	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KFU76_RS13675 (2832263)	2832263..2833192	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KFU76_RS13680 (2833522)	2833522..2833764	-	243	WP_001087409.1	protein YiiF	-
KFU76_RS13685 (2833984)	2833984..2834202	-	219	WP_001315930.1	CopG family transcriptional regulator	-
KFU76_RS13690 (2834807)	2834807..2835097	-	291	WP_000356397.1	NadS family protein	Antitoxin
KFU76_RS13695 (2835098)	2835098..2835409	-	312	WP_000897305.1	hypothetical protein	Toxin
KFU76_RS13700 (2835638)	2835638..2836546	+	909	WP_001162704.1	alpha/beta hydrolase	-
KFU76_RS13705 (2836610)	2836610..2837551	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KFU76_RS13710 (2837596)	2837596..2838033	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KFU76_RS13715 (2838030)	2838030..2838902	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KFU76_RS13720 (2838896)	2838896..2839495	-	600	WP_001295269.1	glucose-1-phosphatase	-
KFU76_RS13725 (2839594)	2839594..2840379	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T201177 WP_000897305.1 NZ_CP074028:c2835409-2835098 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T201177 NZ_CP098229:1890093-1890347 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT201177 WP_000356397.1 NZ_CP074028:c2835097-2834807 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT201177 NZ_CP098229:1889845-1890096 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7