Detailed information of TA system TA201160

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 110644..111479
• Replicon: chromosome
• Accession: NZ_CP074028
• Organism: Escherichia coli strain PK13

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1X9TM58
• Locus tag: KFU76_RS00520
• Protein ID: WP_000854761.1
• Coordinates: 110644..111021 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: KFU76_RS00525
• Protein ID: WP_001295723.1
• Coordinates: 111111..111479 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU76_RS00480 (106033)	106033..106398	-	366	WP_001282181.1	EutP/PduV family microcompartment system protein	-
KFU76_RS00485 (106669)	106669..107058	-	390	WP_257171079.1	transposase	-
KFU76_RS00490 (107247)	107247..107471	-	225	Protein_97	transposase	-
KFU76_RS00495 (107552)	107552..107956	+	405	WP_000839179.1	transposase	-
KFU76_RS00500 (107953)	107953..108300	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
KFU76_RS00505 (108349)	108349..109888	+	1540	Protein_100	IS66-like element ISEc22 family transposase	-
KFU76_RS00510 (110319)	110319..110399	-	81	Protein_101	hypothetical protein	-
KFU76_RS00515 (110499)	110499..110647	-	149	Protein_102	DUF5983 family protein	-
KFU76_RS00520 (110644)	110644..111021	-	378	WP_000854761.1	TA system toxin CbtA family protein	Toxin
KFU76_RS00525 (111111)	111111..111479	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFU76_RS00530 (111642)	111642..111863	-	222	WP_000692345.1	DUF987 domain-containing protein	-
KFU76_RS00535 (111926)	111926..112402	-	477	WP_001186711.1	RadC family protein	-
KFU76_RS00540 (112417)	112417..112890	-	474	WP_000855059.1	antirestriction protein	-
KFU76_RS00545 (113232)	113232..114050	-	819	WP_001234729.1	DUF932 domain-containing protein	-
KFU76_RS00550 (114326)	114326..115867	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	107552..116628	9076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14205.25 Da
• Isoelectric Point: 7.3249

>T201160 WP_000854761.1 NZ_CP074028:c111021-110644 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T201160 NZ_CP098227:3548150-3548368 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT201160 WP_001295723.1 NZ_CP074028:c111479-111111 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT201160 NZ_CP098227:3547750-3548124 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A1X9TM58

Antitoxin

• AlphaFold DB: S1NM52