Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 584824..585623 Replicon chromosome
Accession NZ_CP074001
Organism Escherichia coli strain PM4

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU80_RS02885 Protein ID WP_000347273.1
Coordinates 584824..585288 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU80_RS02890 Protein ID WP_001307405.1
Coordinates 585288..585623 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU80_RS02855 (579825) 579825..580259 - 435 WP_000948842.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU80_RS02860 (580277) 580277..581155 - 879 WP_094314169.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU80_RS02865 (581145) 581145..581924 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU80_RS02870 (581935) 581935..582408 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU80_RS02875 (582431) 582431..583711 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU80_RS02880 (583960) 583960..584769 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU80_RS02885 (584824) 584824..585288 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU80_RS02890 (585288) 585288..585623 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU80_RS02895 (585772) 585772..587343 - 1572 WP_001273753.1 galactarate dehydratase -
KFU80_RS02900 (587718) 587718..589052 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU80_RS02905 (589068) 589068..589838 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T201007 WP_000347273.1 NZ_CP074001:c585288-584824 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T201007 NZ_CP098208:c55166-54888 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT201007 WP_001307405.1 NZ_CP074001:c585623-585288 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT201007 NZ_CP098208:c55435-55166 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References