Detailed information of TA system TA200928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3567541..3568378
• Replicon: chromosome
• Accession: NZ_CP073984
• Organism: Escherichia coli strain MB15

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KFU92_RS17565
• Protein ID: WP_000227784.1
• Coordinates: 3567836..3568378 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KFU92_RS17560
• Protein ID: WP_001297137.1
• Coordinates: 3567541..3567852 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU92_RS17535 (3562561)	3562561..3563508	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KFU92_RS17540 (3563530)	3563530..3565521	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KFU92_RS17545 (3565511)	3565511..3566125	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KFU92_RS17550 (3566125)	3566125..3566454	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KFU92_RS17555 (3566466)	3566466..3567356	+	891	WP_000971336.1	heme o synthase	-
KFU92_RS17560 (3567541)	3567541..3567852	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KFU92_RS17565 (3567836)	3567836..3568378	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KFU92_RS17570 (3568434)	3568434..3569369	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KFU92_RS17575 (3569777)	3569777..3571141	+	1365	WP_001000978.1	MFS transporter	-
KFU92_RS17580 (3571269)	3571269..3571760	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KFU92_RS17585 (3571928)	3571928..3572839	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T200928 WP_000227784.1 NZ_CP073984:3567836-3568378 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T200928 NZ_CP098197:4336972-4337229 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT200928 WP_001297137.1 NZ_CP073984:3567541-3567852 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT200928 NZ_CP098197:4337241-4337786 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3