Detailed information of TA system TA200907

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4476873..4477705
• Replicon: chromosome
• Accession: NZ_CP073981
• Organism: Escherichia coli strain MB19

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: F4TJC5
• Locus tag: KFU84_RS21745
• Protein ID: WP_001094452.1
• Coordinates: 4476873..4477247 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KFU84_RS21750
• Protein ID: WP_112019398.1
• Coordinates: 4477337..4477705 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU84_RS21710 (4472458)	4472458..4473012	-	555	WP_001001921.1	molecular chaperone YcdY	-
KFU84_RS21715 (4473036)	4473036..4473773	-	738	WP_000283664.1	zinc-binding phosphatase	-
KFU84_RS21720 (4473828)	4473828..4474766	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
KFU84_RS21730 (4475237)	4475237..4476079	-	843	WP_063082311.1	DUF4942 domain-containing protein	-
KFU84_RS21735 (4476176)	4476176..4476373	-	198	WP_000772035.1	DUF957 domain-containing protein	-
KFU84_RS21740 (4476385)	4476385..4476876	-	492	WP_123000833.1	DUF5983 family protein	-
KFU84_RS21745 (4476873)	4476873..4477247	-	375	WP_001094452.1	TA system toxin CbtA family protein	Toxin
KFU84_RS21750 (4477337)	4477337..4477705	-	369	WP_112019398.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFU84_RS21755 (4477882)	4477882..4478103	-	222	WP_000692300.1	DUF987 domain-containing protein	-
KFU84_RS21760 (4478172)	4478172..4478648	-	477	WP_001186770.1	RadC family protein	-
KFU84_RS21765 (4478664)	4478664..4479137	-	474	WP_075362640.1	antirestriction protein	-
KFU84_RS21770 (4479479)	4479479..4480297	-	819	WP_075362641.1	DUF932 domain-containing protein	-
KFU84_RS21775 (4480414)	4480414..4480609	-	196	Protein_4234	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14014.86 Da
• Isoelectric Point: 7.2920

>T200907 WP_001094452.1 NZ_CP073981:c4477247-4476873 [Escherichia coli]
MNTLPDTHVREASRCSSPVTIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSTDILRARRATGLMTRDNYRTVNNITLGKHPEAK

Nucleotide

• Length: 375 bp

>T200907 NZ_CP098195:c4875679-4875368 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13660.52 Da
• Isoelectric Point: 7.3712

>AT200907 WP_112019398.1 NZ_CP073981:c4477705-4477337 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT200907 NZ_CP098195:c4875367-4875077 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A7Z7YIM6