Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2481740..2482394 Replicon chromosome
Accession NZ_CP073981
Organism Escherichia coli strain MB19

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU84_RS11960 Protein ID WP_000244777.1
Coordinates 2481987..2482394 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU84_RS11955 Protein ID WP_000354046.1
Coordinates 2481740..2482006 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU84_RS11930 (2476909) 2476909..2477652 + 744 WP_000951964.1 SDR family oxidoreductase -
KFU84_RS11935 (2477709) 2477709..2479142 - 1434 WP_032181793.1 6-phospho-beta-glucosidase BglA -
KFU84_RS11940 (2479187) 2479187..2479498 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KFU84_RS11945 (2479662) 2479662..2480321 + 660 WP_000250274.1 hemolysin III family protein -
KFU84_RS11950 (2480517) 2480517..2481497 - 981 WP_000886074.1 tRNA-modifying protein YgfZ -
KFU84_RS11955 (2481740) 2481740..2482006 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU84_RS11960 (2481987) 2481987..2482394 + 408 WP_000244777.1 protein YgfX Toxin
KFU84_RS11965 (2482434) 2482434..2482955 - 522 WP_001055874.1 flavodoxin FldB -
KFU84_RS11970 (2483067) 2483067..2483963 + 897 WP_000806628.1 site-specific tyrosine recombinase XerD -
KFU84_RS11975 (2483988) 2483988..2484698 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU84_RS11980 (2484704) 2484704..2486437 + 1734 WP_000813208.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T200892 WP_000244777.1 NZ_CP073981:2481987-2482394 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200892 NZ_CP098195:1198794-1199129 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200892 WP_000354046.1 NZ_CP073981:2481740-2482006 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200892 NZ_CP098195:1198546-1198794 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGAGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References