Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 616427..617226 Replicon chromosome
Accession NZ_CP073962
Organism Escherichia coli strain MB31

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag KFU79_RS03000 Protein ID WP_000347275.1
Coordinates 616427..616891 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU79_RS03005 Protein ID WP_001307405.1
Coordinates 616891..617226 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU79_RS02970 (611428) 611428..611862 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU79_RS02975 (611880) 611880..612758 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU79_RS02980 (612748) 612748..613527 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU79_RS02985 (613538) 613538..614011 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU79_RS02990 (614034) 614034..615314 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU79_RS02995 (615563) 615563..616372 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU79_RS03000 (616427) 616427..616891 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU79_RS03005 (616891) 616891..617226 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU79_RS03010 (617375) 617375..618946 - 1572 WP_001273752.1 galactarate dehydratase -
KFU79_RS03015 (619321) 619321..620655 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU79_RS03020 (620671) 620671..621441 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T200827 WP_000347275.1 NZ_CP073962:c616891-616427 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T200827 NZ_CP098183:c1972362-1972255 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200827 WP_001307405.1 NZ_CP073962:c617226-616891 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200827 NZ_CP098183:1972409-1972475 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References