Detailed information of TA system TA200826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 12368..12930
• Replicon: plasmid pYLMB46b
• Accession: NZ_CP073960
• Organism: Escherichia coli strain MB46

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: KFU88_RS24550
• Protein ID: WP_223232864.1
• Coordinates: 12368..12616 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: KFU88_RS24555
• Protein ID: WP_023338085.1
• Coordinates: 12661..12930 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU88_RS24520 (KFU88_24265)	7818..8399	+	582	WP_029396071.1	hypothetical protein	-
KFU88_RS24525 (KFU88_24270)	8411..8920	+	510	WP_023338081.1	hypothetical protein	-
KFU88_RS24530 (KFU88_24275)	9080..10192	+	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
KFU88_RS24535 (KFU88_24280)	10386..10541	-	156	WP_001352007.1	type I toxin-antitoxin system toxin HokD	-
KFU88_RS24540 (KFU88_24285)	10846..11622	-	777	Protein_10	transposase	-
KFU88_RS24550 (KFU88_24295)	12368..12616	-	249	WP_223232864.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFU88_RS24555 (KFU88_24300)	12661..12930	-	270	WP_023338085.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
KFU88_RS24560 (KFU88_24305)	13894..14199	+	306	Protein_14	IS6 family transposase	-
KFU88_RS24565 (KFU88_24310)	14199..14804	-	606	WP_000509966.1	recombinase family protein	-
KFU88_RS24570 (KFU88_24315)	14899..17796	+	2898	WP_086660360.1	Tn3-like element Tn5403 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	wbbO / wbbN / glf / wbbM / wzt / wzm	1..44896	44896
	-	inside	IScluster/Tn	-	-	9080..19492	10412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (1-76)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9475.90 Da
• Isoelectric Point: 7.3132

>T200826 WP_223232864.1 NZ_CP073960:c12616-12368 [Escherichia coli]
IREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSQWGKVYILRVLHTTQKWVMLP
TY

Nucleotide

• Length: 249 bp

>T200826 NZ_CP098183:c1971826-1971719 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10227.62 Da
• Isoelectric Point: 10.5807

>AT200826 WP_023338085.1 NZ_CP073960:c12930-12661 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQNNTWFRNQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT200826 NZ_CP098183:1971879-1971940 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT