Detailed information of TA system TA200823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4232351..4232949
• Replicon: chromosome
• Accession: NZ_CP073958
• Organism: Escherichia coli strain MB46

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: KFU88_RS20905
• Protein ID: WP_053291281.1
• Coordinates: 4232351..4232728 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: KFU88_RS20910
• Protein ID: WP_001603498.1
• Coordinates: 4232728..4232949 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU88_RS20875 (4227575)	4227575..4227913	+	339	WP_000883400.1	divalent cation tolerance protein CutA	-
KFU88_RS20880 (4227889)	4227889..4229586	+	1698	WP_000068902.1	protein-disulfide reductase DsbD	-
KFU88_RS20885 (4229623)	4229623..4230198	+	576	WP_001188520.1	transcriptional regulator	-
KFU88_RS20895 (4230578)	4230578..4231840	+	1263	WP_257187912.1	integrase arm-type DNA-binding domain-containing protein	-
KFU88_RS20900 (4232026)	4232026..4232241	+	216	Protein_4084	transposase	-
KFU88_RS20905 (4232351)	4232351..4232728	-	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KFU88_RS20910 (4232728)	4232728..4232949	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFU88_RS20915 (4233134)	4233134..4234153	-	1020	WP_257187913.1	fimbrial protein	-
KFU88_RS20920 (4234165)	4234165..4236651	-	2487	WP_063101695.1	fimbrial biogenesis outer membrane usher protein	-
KFU88_RS20925 (4236669)	4236669..4237349	-	681	WP_053270475.1	fimbrial assembly chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4229623..4263195	33572
	-	flank	IS/Tn	-	-	4232026..4232307	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T200823 WP_053291281.1 NZ_CP073958:c4232728-4232351 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T200823 NZ_CP098183:c1124699-1124157 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT200823 WP_001603498.1 NZ_CP073958:c4232949-4232728 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT200823 NZ_CP098183:c1124994-1124683 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0