Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1312130..1312755 Replicon chromosome
Accession NZ_CP073953
Organism Escherichia coli strain MB49

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KFU90_RS06360 Protein ID WP_000911329.1
Coordinates 1312357..1312755 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KFU90_RS06355 Protein ID WP_000450524.1
Coordinates 1312130..1312357 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU90_RS06330 (1307932) 1307932..1308402 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
KFU90_RS06335 (1308402) 1308402..1308974 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KFU90_RS06340 (1309120) 1309120..1309998 + 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
KFU90_RS06345 (1310015) 1310015..1311049 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KFU90_RS06350 (1311262) 1311262..1311975 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KFU90_RS06355 (1312130) 1312130..1312357 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KFU90_RS06360 (1312357) 1312357..1312755 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU90_RS06365 (1312902) 1312902..1313765 + 864 WP_001267498.1 neutral zinc metallopeptidase -
KFU90_RS06370 (1313780) 1313780..1315795 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
KFU90_RS06375 (1315869) 1315869..1316567 + 699 WP_000679823.1 esterase -
KFU90_RS06380 (1316677) 1316677..1316877 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T200773 WP_000911329.1 NZ_CP073953:1312357-1312755 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T200773 NZ_CP098169:c1818139-1817756 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT200773 WP_000450524.1 NZ_CP073953:1312130-1312357 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT200773 NZ_CP098169:c1818381-1818139 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References