Detailed information of TA system TA200742

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4311240..4312075
• Replicon: chromosome
• Accession: NZ_CP073946
• Organism: Escherichia coli strain MB51

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: KFU68_RS21200
• Protein ID: WP_000854821.1
• Coordinates: 4311698..4312075 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: KFU68_RS21195
• Protein ID: WP_001285610.1
• Coordinates: 4311240..4311608 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU68_RS21160 (4307217)	4307217..4307897	+	681	WP_077064622.1	WYL domain-containing protein	-
KFU68_RS21165 (4308045)	4308045..4308722	+	678	WP_063073422.1	hypothetical protein	-
KFU68_RS21170 (4308728)	4308728..4308880	+	153	WP_001696589.1	DUF905 family protein	-
KFU68_RS21175 (4308981)	4308981..4309799	+	819	WP_001234688.1	DUF932 domain-containing protein	-
KFU68_RS21180 (4309881)	4309881..4310360	+	480	WP_000860074.1	antirestriction protein	-
KFU68_RS21185 (4310376)	4310376..4310852	+	477	WP_001366830.1	RadC family protein	-
KFU68_RS21190 (4310939)	4310939..4311160	+	222	WP_000692350.1	DUF987 domain-containing protein	-
KFU68_RS21195 (4311240)	4311240..4311608	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFU68_RS21200 (4311698)	4311698..4312075	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KFU68_RS21205 (4312072)	4312072..4312269	+	198	Protein_4147	DUF5983 family protein	-
KFU68_RS21210 (4312297)	4312297..4312494	+	198	WP_023563519.1	DUF957 domain-containing protein	-
KFU68_RS21215 (4312579)	4312579..4313421	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
KFU68_RS21220 (4314171)	4314171..4315709	+	1539	WP_257201879.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T200742 WP_000854821.1 NZ_CP073946:4311698-4312075 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T200742 NZ_CP098162:c100476-99994 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT200742 WP_001285610.1 NZ_CP073946:4311240-4311608 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT200742 NZ_CP098162:c100730-100464 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54