Detailed information of TA system TA200643

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44637..44906
• Replicon: plasmid pYLMB57a
• Accession: NZ_CP073933
• Organism: Escherichia coli strain MB57

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KFU62_RS22795
• Protein ID: WP_001372321.1
• Coordinates: 44781..44906 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	44637..44702 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU62_RS22750 (39665)	39665..39931	+	267	WP_072667006.1	hypothetical protein	-
KFU62_RS22755 (40172)	40172..40378	+	207	WP_000275858.1	hypothetical protein	-
KFU62_RS22760 (40404)	40404..40943	+	540	WP_001825195.1	single-stranded DNA-binding protein	-
KFU62_RS22765 (41006)	41006..41239	+	234	WP_000005990.1	DUF905 family protein	-
KFU62_RS22770 (41305)	41305..43263	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
KFU62_RS22775 (43318)	43318..43752	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
KFU62_RS22780 (43749)	43749..44511	+	763	Protein_52	plasmid SOS inhibition protein A	-
KFU62_RS22785 (44480)	44480..44668	-	189	WP_001299721.1	hypothetical protein	-
- (44637)	44637..44702	+	66	NuclAT_1	-	-
- (44637)	44637..44702	-	66	NuclAT_0	-	Antitoxin
- (44637)	44637..44702	-	66	NuclAT_0	-	Antitoxin
- (44480)	44480..44704	+	225	NuclAT_0	-	-
- (44480)	44480..44704	+	225	NuclAT_0	-	-
- (44480)	44480..44704	+	225	NuclAT_0	-	-
- (44480)	44480..44704	+	225	NuclAT_0	-	-
- (44480)	44480..44704	-	225	NuclAT_0	-	-
KFU62_RS22790 (44690)	44690..44839	+	150	Protein_54	plasmid maintenance protein Mok	-
KFU62_RS22795 (44781)	44781..44906	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
KFU62_RS22800 (45126)	45126..45356	+	231	WP_001426396.1	hypothetical protein	-
KFU62_RS22805 (45354)	45354..45527	-	174	Protein_57	hypothetical protein	-
KFU62_RS22810 (45597)	45597..45803	+	207	WP_000547968.1	hypothetical protein	-
KFU62_RS22815 (45828)	45828..46115	+	288	WP_000107544.1	hypothetical protein	-
KFU62_RS22820 (46233)	46233..47054	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KFU62_RS22825 (47351)	47351..47998	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
KFU62_RS22830 (48275)	48275..48658	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KFU62_RS22835 (48849)	48849..49535	+	687	WP_000332484.1	PAS domain-containing protein	-
KFU62_RS22840 (49630)	49630..49857	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T200643 WP_001372321.1 NZ_CP073933:44781-44906 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T200643 NZ_CP098128:c4901417-4901049 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT200643 NZ_CP073933:c44702-44637 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin