Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 842833..843487 Replicon chromosome
Accession NZ_CP073932
Organism Escherichia coli strain MB57

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU62_RS04130 Protein ID WP_000244777.1
Coordinates 843080..843487 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU62_RS04125 Protein ID WP_000354046.1
Coordinates 842833..843099 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU62_RS04100 (838002) 838002..838745 + 744 WP_000951964.1 SDR family oxidoreductase -
KFU62_RS04105 (838802) 838802..840235 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KFU62_RS04110 (840280) 840280..840591 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KFU62_RS04115 (840755) 840755..841414 + 660 WP_000250274.1 hemolysin III family protein -
KFU62_RS04120 (841610) 841610..842590 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU62_RS04125 (842833) 842833..843099 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU62_RS04130 (843080) 843080..843487 + 408 WP_000244777.1 protein YgfX Toxin
KFU62_RS04135 (843527) 843527..844048 - 522 WP_001055874.1 flavodoxin FldB -
KFU62_RS04140 (844160) 844160..845056 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU62_RS04145 (845081) 845081..845791 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU62_RS04150 (845797) 845797..847530 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T200622 WP_000244777.1 NZ_CP073932:843080-843487 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200622 NZ_CP098123:1961470-1961572 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200622 WP_000354046.1 NZ_CP073932:842833-843099 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200622 NZ_CP098123:c1961579-1961434 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References