Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3769848..3770389 Replicon chromosome
Accession NZ_CP073929
Organism Escherichia coli strain MB64

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KFU91_RS18150 Protein ID WP_000615976.1
Coordinates 3770111..3770389 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KFU91_RS18145 Protein ID WP_000729704.1
Coordinates 3769848..3770108 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU91_RS18125 (3765523) 3765523..3766308 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
KFU91_RS18130 (3766280) 3766280..3767992 + 1713 Protein_3546 flagellar biosynthesis protein FlhA -
KFU91_RS18135 (3768216) 3768216..3768713 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
KFU91_RS18140 (3768889) 3768889..3769662 - 774 WP_015675129.1 C40 family peptidase -
KFU91_RS18145 (3769848) 3769848..3770108 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KFU91_RS18150 (3770111) 3770111..3770389 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KFU91_RS18155 (3770545) 3770545..3771285 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KFU91_RS18160 (3771256) 3771256..3772023 - 768 WP_000333379.1 class II glutamine amidotransferase -
KFU91_RS18165 (3772229) 3772229..3772807 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T200614 WP_000615976.1 NZ_CP073929:3770111-3770389 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T200614 NZ_CP098040:c4272031-4271846 [Providencia rettgeri]
GTGCAAAGTTCGGAACTGATAAACATACTGCAAAAAAACGGTTGGAAACTTGAAAGAATAAAAGGAAGTCACCATCAGTT
TTCCCACCCACATTTTTCAATTGTAATTACTGTTCCGCATCCACAAAAAGATTTAAAGATAGGCACACTAAATCACATTT
TGAAGGCTGCGAAACTAAAACATTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT200614 WP_000729704.1 NZ_CP073929:3769848-3770108 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT200614 NZ_CP098040:c4271796-4271383 [Providencia rettgeri]
ATGCTTTATACCGCCTTTATTGAAATTGATGATGATGGCAGCGCAAGTGGCTGGTTTCCTGATATTGAAGGATGTACATT
CGCGGGCAGCAATATAGAAGAGGCTTACGCAGAAGCAAAATCAGCAATTGATGCTCATTTTGAATTATTGAGTGAAAAAG
GCTTTGAGATCCCATTATCGAAATCTCAGCAAGCGCTATTGAACCCAATACAACCTGAATATGCGCATGGGATATGGTTA
TTTGTGGATGTCGATATGGATAAATACGATGGCCGTACTGAGCGTATTAATATCACCTTGCCGCATCGTTTATTACATCG
TATTGATGCTTTAGTGAAGGTTAATCCTGAATATGGTAGCCGTAGTGGTTTTATTGCTGTTGCAGCTCGTAAAGAATTAA
AAAAAACCGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References