Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2574235..2574761 Replicon chromosome
Accession NZ_CP073929
Organism Escherichia coli strain MB64

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KFU91_RS12270 Protein ID WP_000323025.1
Coordinates 2574235..2574522 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KFU91_RS12275 Protein ID WP_000534858.1
Coordinates 2574522..2574761 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU91_RS12235 (2569625) 2569625..2569840 - 216 WP_000839565.1 phage lysis protein EssD -
KFU91_RS12240 (2570092) 2570092..2570466 - 375 WP_001348108.1 cell envelope integrity TolA C-terminal domain-containing protein -
KFU91_RS12245 (2570638) 2570638..2570898 - 261 Protein_2397 cell envelope integrity protein TolA -
KFU91_RS12250 (2572111) 2572111..2572653 - 543 WP_000640161.1 DUF1133 family protein -
KFU91_RS12255 (2572650) 2572650..2572940 - 291 WP_000247763.1 DUF1364 domain-containing protein -
KFU91_RS12260 (2572940) 2572940..2573539 - 600 WP_025670565.1 DUF1367 family protein -
KFU91_RS12265 (2574008) 2574008..2574163 - 156 WP_000813269.1 type I toxin-antitoxin system toxin HokD -
KFU91_RS12270 (2574235) 2574235..2574522 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KFU91_RS12275 (2574522) 2574522..2574761 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KFU91_RS12280 (2574786) 2574786..2575091 + 306 WP_071600497.1 protein YdfV -
KFU91_RS12285 (2575294) 2575294..2575626 + 333 WP_001349760.1 FlxA-like family protein -
KFU91_RS12290 (2576063) 2576063..2576212 - 150 WP_011443592.1 protein YdfW -
KFU91_RS12295 (2576333) 2576333..2577382 - 1050 Protein_2407 ISNCY family transposase -
KFU91_RS12300 (2578199) 2578199..2579398 + 1200 WP_001117227.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2535737..2577403 41666


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T200609 WP_000323025.1 NZ_CP073929:c2574522-2574235 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T200609 NZ_CP098040:2201941-2203260 [Providencia rettgeri]
ATGGCGACTTTGCATGTATACATGAATGGTTACTTGGTTGGGGATTTGATTCGTTCAAGTACTGGTGCTCACCAATTTAC
TTATAATAGTCACTGGTTGGAAACATCAGGTGCTCGACCTATTTCTTTATCAATGCCACTTAGGCACCAAGAATATCGAG
GAGACGAAGTTTATAATTTTTTTGATAATCTCCTACCGGACAATATTGAGATACGGAATCGAATTGTTAGTCGTTATCAA
GCGGATTCAAATCAACCTTTCGATTTATTATCTAAGATTGGCCAGGATAGTGTGGGGGCTTTACAACTCATTCTAGATGG
GCAAAGAGTCAGTAATATCAGAAAAATTGAATATAAACCCTTATCTAATAAGCAACTAGAAAAAATATTAACAGGGTATA
AAGCGAATGCACCTTTAGGCATGATTGAAGATGAAGATGACTTTAGGATCTCACTTGCGGGCGCTCAAGAGAAAACCGCA
TTACTCTATTTAGATGGGCATTGGTGCTTACCTCAGAATGCGACTCCAACTACCCATATTATTAAACTTCCCATTGGTAA
AATAGAAAGCCATTCTTATTCGATAGATATGTCGGATAGTGTAGAAAATGAATATTTATGTTTATTGATAGCAAAAGAGT
TTGGCCTAGATGTGCCCTATAGCTTTATTTTAAATGTAGGTGAAATAAAGGCGTTAGCGGTTGAACGTTTTGACCGTAAA
TATTCTTCTGATTCCTCGTGGATTATGCGTTTACCACAAGAAGATTTTTGTCAAACATTGAATATTTCACCGGCAAGAAA
ATATCAAAATCATGGTGGACCGGGTATTTCTGAAATTATGAATTACTTATTAGGTTCAATAAATGCGGAGAAAGATAGAT
ATCAGTTTATGAAGAGCCAGGTTTTGTTTTGGTTACTTGCAGCGACGGATGGCCATGCTAAAAATTTTTCATTATTTATT
GAACCTGAAGGCCGTTATCAGTTAACACCTTTTTACGATATTTTGTCCATGTATCCTAGTTATGGGGGGAGGGGAATTAA
CCCTAGAGATGCCAAATTGGCTATGGGGCTGAAGGGAACAAGGGGGATGAAATACAATATAGAGCAAATTTTCCCAAGAC
ATTTTTTTTCAACGGCCAAAGCCGTTGGATTTGATCGTGCTGAGATGGAAAAAATCTTAATTGAATTTGGTGAGCAAATG
GAAAGTGTTATTAAGAAAGTGAGAGAACAATTACCCACAGATTTCCCTGAGCATATCGCAGACTCAATTCTATCCGGATT
ACATCACAAAGCAGCGAGATTAAATAAAGGCTGGGACTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT200609 WP_000534858.1 NZ_CP073929:c2574761-2574522 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT200609 NZ_CP098040:2201684-2201941 [Providencia rettgeri]
ATGAAAGTGACGAATACCCGACAACTTAGTGCTTATATGAAGGATGTCAGAGAGACGAAAAAGTTATCTCAAAGCAAAGT
CGCTAGCAAAGTAGGTATTCGGCAAGATACTGTTTCTAGCTTTGAACTCTCTCCTGACTCTACCAAATTAGAAACCTTTT
TTAAGATTTTATCTGCCCTCAATTTAGAACTTGATATTAAACCGCGAAATGAAAGCGGCTCATCAAATTCAGACTCCGGA
TGGAAGGAGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References