Detailed information of TA system TA200607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2369545..2371133
• Replicon: chromosome
• Accession: NZ_CP073929
• Organism: Escherichia coli strain MB64

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: KFU91_RS11345
• Protein ID: WP_001125439.1
• Coordinates: 2369811..2371133 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KFU91_RS11340
• Protein ID: WP_001301023.1
• Coordinates: 2369545..2369811 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU91_RS11325 (2364646)	2364646..2365364	+	719	WP_257199188.1	IS1-like element IS1A family transposase	-
KFU91_RS11330 (2365715)	2365715..2367811	+	2097	WP_257199190.1	hypothetical protein	-
KFU91_RS11335 (2367772)	2367772..2369322	+	1551	WP_024203003.1	autotransporter outer membrane beta-barrel domain-containing protein	-
KFU91_RS11340 (2369545)	2369545..2369811	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KFU91_RS11345 (2369811)	2369811..2371133	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KFU91_RS11350 (2371384)	2371384..2372784	+	1401	WP_001409388.1	site-specific integrase	-
KFU91_RS11355 (2372768)	2372768..2374318	+	1551	WP_000086931.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2363967..2397797	33830
	-	flank	IS/Tn	-	-	2364861..2365364	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T200607 WP_001125439.1 NZ_CP073929:2369811-2371133 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T200607 NZ_CP098040:c1024662-1024252 [Providencia rettgeri]
GTGGTCCTGTGGAAATCTAATCTCTCCATTTCATGGAAAACCCAACTGTTCTCAACCTGTGTTCATGGGGTCATTGGGAT
GTTTTTACTATTGGCTCCATGGTCCCCTGGTAACTCAATGATATGGTTGCCGCTATTAGTGGTTGTCGTTGCGAGTTGGG
CAAAGAGCCAGAAAAATATCAGTAAAATAAAAGGGGTTGCTGTACTGGTTAATGGCAATAAGGTTCAGTGGAAGAAAAAT
GAATGGAGAATACTGAAAGCACCTTGGCTTACCCGCTATGGTATATTACTGACACTAGAAGCGTTACAAGGTAAACCACA
AAAGCTCCATTTGTGGGTTGCAAAAGACGCATTGTCGGAAGAGAATTGGCGTAATTTAAACCAATTATTGCTGCAATACC
CTGATATTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT200607 WP_001301023.1 NZ_CP073929:2369545-2369811 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT200607 NZ_CP098040:c1024909-1024643 [Providencia rettgeri]
ATGGATATTGAGAATAAAGCGCGAATTCATTGGGCATGCCGCCGTGGTATGCGTGAATTAGATATTTCAATCATGCCGTT
TTTTGAGTATGAATATGATGACCTAACAACTCACGATAAGCAGGTATTTGTCCGTTTATTAGAATGTGCGGACCCTGATT
TATTCAATTGGCTAATGAATCACGGTCGTCCAGATGATGACGAACTTTTTCGTATGATTAAACTGATTCAGGAAAAAAAT
AAAAATCGTGGTCCTGTGGAAATCTAA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU