Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 637511..638310 Replicon chromosome
Accession NZ_CP073929
Organism Escherichia coli strain MB64

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag KFU91_RS03085 Protein ID WP_000347273.1
Coordinates 637511..637975 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KFU91_RS03090 Protein ID WP_001307405.1
Coordinates 637975..638310 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU91_RS03055 (632512) 632512..632946 - 435 WP_000948814.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KFU91_RS03060 (632964) 632964..633842 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KFU91_RS03065 (633832) 633832..634611 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KFU91_RS03070 (634622) 634622..635095 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KFU91_RS03075 (635118) 635118..636398 - 1281 WP_104768934.1 tagatose-bisphosphate aldolase subunit KbaZ -
KFU91_RS03080 (636647) 636647..637456 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KFU91_RS03085 (637511) 637511..637975 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KFU91_RS03090 (637975) 637975..638310 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KFU91_RS03095 (638459) 638459..640030 - 1572 WP_001273753.1 galactarate dehydratase -
KFU91_RS03100 (640405) 640405..641739 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KFU91_RS03105 (641755) 641755..642525 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T200593 WP_000347273.1 NZ_CP073929:c637975-637511 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T200593 NZ_CP098030:c3825324-3824968 [Serratia ureilytica]
ATGATCTTTTTGACCGCAGAGGATATTGCGGAGTTCAATGCTGAAATTGTGCCTCACGGCCGCCAGGAAGGCAGCAAAGT
TGAGGCTGTAGCCAACCGCGTGCTCAATGCTTACCACTACGAGAACGTCACAGACGTATACCGCTTGGCCGCGCTCTATC
TCATCGCCATCAGCCACGGTCACATTTTTCTTGATGGCAACAAACGTACGGCCTTCCAAAGCATGGCGCTATTTCTCGGT
ATTAATGGCATTGCACTGCGGGAAGACGCAGAACTGGTCGAAATTACGGTTGAAGCAGCTCAAGGCCGCCTCAACGTAGA
ACAAGCCGCTGAACACTTACGCCGGCTAACCGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT200593 WP_001307405.1 NZ_CP073929:c638310-637975 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT200593 NZ_CP098030:c3825530-3825321 [Serratia ureilytica]
ATGACTACGATTAGCTACACGGCGGCGAGAAACAATTTGGCCGAGGTGCTTTTGGAAGCGCAAAAACAGCCGGTGGAAGT
GACGCGCCGCGGACACGATGAGGTTTACATCATCAGTAAGGCCGACTATGAGGTTTTGGTCAAAGCCAAAGTGAAAGCGC
GCATTCAGGCTAAACACGCGGATACGATTAAGGCGCTGGCGAATAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References