Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1411897..1412522 Replicon chromosome
Accession NZ_CP073924
Organism Escherichia coli strain MB165

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KFU71_RS06895 Protein ID WP_000911329.1
Coordinates 1412124..1412522 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KFU71_RS06890 Protein ID WP_000450524.1
Coordinates 1411897..1412124 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU71_RS06865 (1407699) 1407699..1408169 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
KFU71_RS06870 (1408169) 1408169..1408741 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KFU71_RS06875 (1408887) 1408887..1409765 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
KFU71_RS06880 (1409782) 1409782..1410816 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KFU71_RS06885 (1411029) 1411029..1411742 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KFU71_RS06890 (1411897) 1411897..1412124 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KFU71_RS06895 (1412124) 1412124..1412522 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU71_RS06900 (1412669) 1412669..1413532 + 864 WP_001267498.1 neutral zinc metallopeptidase -
KFU71_RS06905 (1413547) 1413547..1415562 + 2016 WP_000829295.1 tRNA cytosine(34) acetyltransferase TmcA -
KFU71_RS06910 (1415636) 1415636..1416334 + 699 WP_000679823.1 esterase -
KFU71_RS06915 (1416444) 1416444..1416644 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T200545 WP_000911329.1 NZ_CP073924:1412124-1412522 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T200545 NZ_CP097896:989028-989975 [Pectobacterium actinidiae]
ATGGATGATTTAACGAAGCTATTCCCAGATGTTGCTGATGCTCTGGGCATTGAATCAGTTGCAATTGTAGAGAAAGATTA
TTACATCGTTGAGTTATTGCGATTGCTTCAGCCTCTGGCATTTGACACGCATCAACTGGTTTTTGCCGGTGGAACGGCAT
TGTCAAAAGCGGGGATATCTTTGAACAGGATGTCAGAAGACGTGGACATTAAGCTTGTCCCCGTCGCCGGTTTTTCCCTG
CAATATTCCAGAAATCAACGTAAAAATATCCGTAAAGATATTATTAAGACCATCACCGAAACGATTGTAGCTTCAGGTAT
GTTTAGCATGGATGAGGAGTACCCAAAGGTTACTCGTGATGAAAACCGCTATAATGAGATTCCGGTGCGTTACCCGCAGA
AATATGCACAAGCTCCTTGCTTAAGACCATTTATCAAATTGGAATTAATGGAAACGGATTTGCTTGAGGCTGCGGTTAAC
CGTGATATTAGCTCATTGGTAATAGATCTAACAAAGAAAGGTGAAGTAGTGAGTGCTTTTCCCTGTGCAAGTATCGACAG
CACTCAGGCTGAAAAGCTGATTTCGATGATGCGCAGAACGGCTGCAAGCTTGCGTGATGTGGAACGTGCAGTGGATGAGT
CACTGGTACGTCATATTTACGACAATTTTTGTATTGTCAGAGAGAAAGGGGCTGATGTTCCGGTTTTGAAACGCTTTGTT
CAGGAATGCATTAAGCAGGATATTGAACGTTACGGTAACCAATATCCTGCGTTCAGCGAAGCACCTGTTGAGGAACTCAA
AATGGGGCTGGAAGAGTTGGGAAGCAATTCTGTTTATGAAGTCCGCTATCAGCAGTTTGTCACTCCAATGGTGTTTGGTG
GAACCCAGATTTCATGGGAAGAGGCCTATTCCTGTTTTCGCCAAACTGCGTTAGAAGTGCTGCAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT200545 WP_000450524.1 NZ_CP073924:1411897-1412124 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT200545 NZ_CP097896:988640-989035 [Pectobacterium actinidiae]
ATGACTACAAAAGCTCGTATTCAGTCGCGGCTGAAACGCTCGAAGCGTTATGTTTTCACTCGTGACGACTTCAAAGATCT
TGCTGGCTACGATCAAGTTGGCAGAGCGCTGCGTGAACTGGTGAAAGAGGGCCTACTGCTGAAAGTAGGTTATGGTGTCT
ATACCAAAGCGCGGAAAAATGTGATTACGGGTAGGTTAATGCCCGCATCTCCCGGTGGTTCTGCTGCGGTGGTGATCGAA
GCGCTGGAGCGCTTGAAAGTGGACTATCAACTAGCTGATGCAGCAAGTGCTTACAATAGCGGCAAGTCAACTCAAGTTCC
AGCGTCAATGGAAATCAAAACTTCACCACGTTTTAAGAGAACATTGTCAGTAGGGAACAGCAAACTAAATGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References