Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 52615..53258 Replicon plasmid unnamed_1
Accession NZ_CP073792
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2010_71621

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag KFO61_RS26280 Protein ID WP_000754566.1
Coordinates 52842..53258 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag KFO61_RS26275 Protein ID WP_001261276.1
Coordinates 52615..52845 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFO61_RS26250 (KFO61_26250) 47682..48859 + 1178 Protein_46 IS3 family transposase -
KFO61_RS26255 (KFO61_26255) 49241..50026 - 786 WP_050485696.1 site-specific integrase -
KFO61_RS26260 (KFO61_26260) 50072..50593 - 522 WP_094313782.1 hypothetical protein -
KFO61_RS26265 (KFO61_26265) 50651..51043 - 393 WP_170933338.1 hypothetical protein -
KFO61_RS26270 (KFO61_26270) 51080..52024 - 945 WP_094313783.1 hypothetical protein -
KFO61_RS26275 (KFO61_26275) 52615..52845 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KFO61_RS26280 (KFO61_26280) 52842..53258 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
KFO61_RS26285 (KFO61_26285) 53293..53538 + 246 WP_065801591.1 hypothetical protein -
KFO61_RS26290 (KFO61_26290) 53602..53856 + 255 WP_095153353.1 hypothetical protein -
KFO61_RS26295 (KFO61_26295) 54413..55419 + 1007 Protein_55 IS110 family transposase -
KFO61_RS26300 (KFO61_26300) 55698..55946 + 249 WP_065801593.1 hypothetical protein -
KFO61_RS26305 (KFO61_26305) 55951..56523 + 573 WP_094309628.1 hypothetical protein -
KFO61_RS26310 (KFO61_26310) 56554..57048 + 495 WP_094313750.1 DNA-binding protein -
KFO61_RS26315 (KFO61_26315) 57109..57312 + 204 WP_004150739.1 HHA domain-containing protein -
KFO61_RS26320 (KFO61_26320) 57361..57570 + 210 WP_094308932.1 hypothetical protein -
KFO61_RS26325 (KFO61_26325) 57600..57908 - 309 Protein_61 integrase core domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iutA / iucD / iucC / iucB / iucA / cseA 1..155175 155175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T200306 WP_000754566.1 NZ_CP073792:52842-53258 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T200306 NZ_CP097808:c5357100-5356717 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT200306 WP_001261276.1 NZ_CP073792:52615-52845 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT200306 NZ_CP097808:c5357342-5357100 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References