Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 2785165..2785687 Replicon chromosome
Accession NZ_CP073711
Organism Lacticaseibacillus rhamnosus strain X253

Toxin (Protein)


Gene name yoeB Uniprot ID C2JUK2
Locus tag KEM50_RS13000 Protein ID WP_005687756.1
Coordinates 2785427..2785687 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID C2JUK3
Locus tag KEM50_RS12995 Protein ID WP_005687754.1
Coordinates 2785165..2785434 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEM50_RS12980 (KEM50_12980) 2780652..2782520 - 1869 WP_014571687.1 HTH domain-containing protein -
KEM50_RS12985 (KEM50_12985) 2782547..2783347 - 801 WP_005690776.1 SDR family oxidoreductase -
KEM50_RS12990 (KEM50_12990) 2783953..2784969 - 1017 WP_019728331.1 IS30 family transposase -
KEM50_RS12995 (KEM50_12995) 2785165..2785434 + 270 WP_005687754.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
KEM50_RS13000 (KEM50_13000) 2785427..2785687 + 261 WP_005687756.1 Txe/YoeB family addiction module toxin Toxin
KEM50_RS13005 (KEM50_13005) 2785898..2786626 - 729 WP_014571689.1 L-ribulose-5-phosphate 4-epimerase -
KEM50_RS13010 (KEM50_13010) 2786823..2787644 + 822 WP_005690770.1 Cof-type HAD-IIB family hydrolase -
KEM50_RS13015 (KEM50_13015) 2787711..2788598 - 888 WP_014571690.1 L-ribulose-5-phosphate 3-epimerase -
KEM50_RS13020 (KEM50_13020) 2788783..2789562 - 780 WP_005690765.1 DeoR/GlpR family DNA-binding transcription regulator -
KEM50_RS13025 (KEM50_13025) 2789630..2790271 - 642 WP_005690763.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10485.91 Da        Isoelectric Point: 9.6577

>T200048 WP_005687756.1 NZ_CP073711:2785427-2785687 [Lacticaseibacillus rhamnosus]
MIKTWTDDAWADYMYWHDQNDKRTIKRINQLIQAIDRDPYKGIGKPEPLRYALTGKWSRRIDQENRIIYSIEKNHINIFA
CRTHYS

Download         Length: 261 bp

>T200048 NZ_CP097693:c114395-113979 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10181.39 Da        Isoelectric Point: 4.6755

>AT200048 WP_005687754.1 NZ_CP073711:2785165-2785434 [Lacticaseibacillus rhamnosus]
MEATNYSDFRRNLKHYMSQVNEDAEPLLVTAKDDDDNVVVMSKHDFDAIEETLYLLSNPKLMAKIKRGDAQIAAGKAKQH
ELLTDFDHD

Download         Length: 270 bp

>AT200048 NZ_CP097693:c114622-114392 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249N526

Antitoxin

Source ID Structure
AlphaFold DB A0A249N594

References