Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2599726..2600377 Replicon chromosome
Accession NZ_CP073711
Organism Lacticaseibacillus rhamnosus strain X253

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag KEM50_RS12095 Protein ID WP_005686631.1
Coordinates 2599726..2600109 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag KEM50_RS12100 Protein ID WP_005686632.1
Coordinates 2600129..2600377 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEM50_RS12090 (KEM50_12090) 2598685..2599212 - 528 WP_005691221.1 QueT transporter family protein -
KEM50_RS12095 (KEM50_12095) 2599726..2600109 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KEM50_RS12100 (KEM50_12100) 2600129..2600377 - 249 WP_005686632.1 antitoxin Antitoxin
KEM50_RS12105 (KEM50_12105) 2600489..2601628 - 1140 WP_005691215.1 alanine racemase -
KEM50_RS12110 (KEM50_12110) 2601615..2601989 - 375 WP_005686634.1 holo-ACP synthase -
KEM50_RS12115 (KEM50_12115) 2602158..2603666 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
KEM50_RS12120 (KEM50_12120) 2603940..2605328 - 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T200047 WP_005686631.1 NZ_CP073711:c2600109-2599726 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T200047 NZ_CP097693:38823-38972 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT200047 WP_005686632.1 NZ_CP073711:c2600377-2600129 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT200047 NZ_CP097693:c38778-38719 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References