Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4311075..4311616 Replicon chromosome
Accession NZ_CP073589
Organism Escherichia coli strain MFDS1006657

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KD154_RS20740 Protein ID WP_000615976.1
Coordinates 4311075..4311353 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KD154_RS20745 Protein ID WP_000729704.1
Coordinates 4311356..4311616 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD154_RS20725 (4308657) 4308657..4309235 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
KD154_RS20730 (4309441) 4309441..4310208 + 768 WP_000333380.1 class II glutamine amidotransferase -
KD154_RS20735 (4310179) 4310179..4310919 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KD154_RS20740 (4311075) 4311075..4311353 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KD154_RS20745 (4311356) 4311356..4311616 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KD154_RS20750 (4311802) 4311802..4312575 + 774 WP_000543895.1 C40 family peptidase -
KD154_RS20755 (4312632) 4312632..4312988 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
KD154_RS20760 (4312981) 4312981..4313259 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KD154_RS20765 (4313364) 4313364..4315076 - 1713 Protein_4083 flagellar biosynthesis protein FlhA -
KD154_RS20770 (4315048) 4315048..4315833 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4301948..4313259 11311


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T199868 WP_000615976.1 NZ_CP073589:c4311353-4311075 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T199868 NZ_CP097646:3901867-3902085 [Klebsiella quasipneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAACAAATATGAACTTTCTGATAATGAGCTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACTATGAATAAGCTGTACGATAAAATCCCGACGTCCGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT199868 WP_000729704.1 NZ_CP073589:c4311616-4311356 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT199868 NZ_CP097646:3901466-3901840 [Klebsiella quasipneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCACAACTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACATCGGCTGTCAATCTCCAGTTAAATGAACTGATCGAGC
ACATTGCCACATTCGCCCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACATTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGGTT
ATTTCGTTGTTTTGTCAACGCCAGCCGGGAAAATCCGGCCAGTCTTTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References