Detailed information of TA system TA199835

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37233..37486
• Replicon: plasmid p00003
• Accession: NZ_CP073380
• Organism: Klebsiella sp. P1927

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KB892_RS30170
• Protein ID: WP_001312851.1
• Coordinates: 37233..37382 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	37427..37486 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB892_RS30135 (32592)	32592..33007	-	416	Protein_44	IS1-like element IS1B family transposase	-
KB892_RS30140 (33256)	33256..33657	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
KB892_RS30145 (33590)	33590..33847	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
KB892_RS30150 (33940)	33940..34593	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
KB892_RS30155 (35532)	35532..36389	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
KB892_RS31350 (36408)	36408..36586	-	179	Protein_49	protein CopA/IncA	-
KB892_RS30165 (36701)	36701..36949	-	249	WP_000083837.1	replication regulatory protein RepA	-
KB892_RS30170 (37233)	37233..37382	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (37427)	37427..37486	+	60	NuclAT_1	-	Antitoxin
- (37427)	37427..37486	+	60	NuclAT_1	-	Antitoxin
- (37427)	37427..37486	+	60	NuclAT_1	-	Antitoxin
- (37427)	37427..37486	+	60	NuclAT_1	-	Antitoxin
KB892_RS30175 (37687)	37687..38019	-	333	WP_152916585.1	hypothetical protein	-
KB892_RS30180 (38081)	38081..38680	-	600	WP_032083981.1	PIN domain-containing protein	-
KB892_RS30185 (39066)	39066..39266	-	201	WP_015059022.1	hypothetical protein	-
KB892_RS30190 (39398)	39398..39958	-	561	WP_000139328.1	fertility inhibition protein FinO	-
KB892_RS30195 (40013)	40013..40759	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
KB892_RS30200 (40779)	40779..40979	-	201	WP_072354025.1	hypothetical protein	-
KB892_RS30205 (41004)	41004..41708	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KB892_RS30210 (41761)	41761..41826	+	66	Protein_59	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / catA2 / blaKPC-2	-	1..162093	162093

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T199835 WP_001312851.1 NZ_CP073380:c37382-37233 [Klebsiella sp. P1927]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T199835 NZ_CP097618:1882197-1882523 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGTG
ATTTACGAAGTCCACCTGGTAATAAATTGGAAAAATTGGAGCCTAAAACGACTGGGCTATATTCTATCAGAGTGAATGTG
AAGTATCGTTTAATATTTAAATATAAAGAAAGTAATTCTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT199835 NZ_CP073380:37427-37486 [Klebsiella sp. P1927]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin