Detailed information of TA system TA199704

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3854665..3855325
• Replicon: chromosome
• Accession: NZ_CP073310
• Organism: Enterobacter cloacae strain CZ862

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: KDU74_RS18760
• Protein ID: WP_178328240.1
• Coordinates: 3854912..3855325 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A5E1AG01
• Locus tag: KDU74_RS18755
• Protein ID: WP_013098615.1
• Coordinates: 3854665..3854931 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDU74_RS18735 (KDU74_18735)	3850074..3851507	-	1434	WP_178328242.1	6-phospho-beta-glucosidase	-
KDU74_RS18740 (KDU74_18740)	3851624..3852355	-	732	WP_020687085.1	MurR/RpiR family transcriptional regulator	-
KDU74_RS18745 (KDU74_18745)	3852621..3853280	+	660	WP_003863433.1	hemolysin III family protein	-
KDU74_RS18750 (KDU74_18750)	3853390..3854370	-	981	WP_178328241.1	tRNA-modifying protein YgfZ	-
KDU74_RS18755 (KDU74_18755)	3854665..3854931	+	267	WP_013098615.1	FAD assembly factor SdhE	Antitoxin
KDU74_RS18760 (KDU74_18760)	3854912..3855325	+	414	WP_178328240.1	protein YgfX	Toxin
KDU74_RS18765 (KDU74_18765)	3855332..3855853	-	522	WP_013098613.1	flavodoxin FldB	-
KDU74_RS18770 (KDU74_18770)	3855955..3856851	+	897	WP_178328239.1	site-specific tyrosine recombinase XerD	-
KDU74_RS18775 (KDU74_18775)	3856880..3857593	+	714	WP_129327561.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KDU74_RS18780 (KDU74_18780)	3857599..3859332	+	1734	WP_178328238.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 16250.24 Da
• Isoelectric Point: 10.9554

>T199704 WP_178328240.1 NZ_CP073310:3854912-3855325 [Enterobacter cloacae]
VVLWQSDLRVSWRSQWMSLLLHGLVAAFVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLSSGMMLRLRQVGGGKCQHLWLAADSMDITEWRELRRMLLQQQPTQE

Nucleotide

• Length: 414 bp

>T199704 NZ_CP097528:c1373938-1373624 [Pseudomonas putida]
ATGATCTTCATCGAGACCCCAGTATTCACCTCCGATCTCAAGGAGCACCTGGATGATGAAGAATACCGGGCTTTGCAGGC
CTATCTGGCCGAGCATCCCGAAGCCGGTTCTCTCCTGGAGGAAACCGGCGGCCTCAGAAAGATCCGCTGGGCAGCCAAGG
GCAAGGGCAAGAGTGGGGGTGTACGAGTGATCTACTACCACGTCACTGCAGCCCACCAGATCCGTATGATTCTGATCTAT
CGCAAGGGCATCGTGGATACCTTGACAAGCAGTCAGAAAGCGCAGCTTCGCGCACTAAACAAAGGGTGGAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10569.05 Da
• Isoelectric Point: 5.1456

>AT199704 WP_013098615.1 NZ_CP073310:3854665-3854931 [Enterobacter cloacae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDEDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT199704 NZ_CP097528:c1373624-1373337 [Pseudomonas putida]
ATGGAAAAGCATCTCTTCGATCGTCTTGTGGAAAGCATGACTCAGATGGATGAGATCGATCGCGGTGAGCGTCAGCCCTC
GCGTGAGTTCCACGTAGACGCACTTCAAGTCAAAAAGATTCGTCAAGCCACTGGCCTTTCCCAGGCTGCGTTTGCCAAAC
GTATCGATGTCGCAGTAGGAACTCTACGGAATTGGGAACAGGGCCGTCGCGAACCTGAAGGACCAGCTCGTGCCTTGCTT
CGAGCGATTCATAACGATCCCGAACATGTACTTGCGGCCTTGAGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AG01