Detailed information of TA system TA199558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 4097683..4098903
• Replicon: chromosome
• Accession: NZ_CP073080
• Organism: Pseudomonas aeruginosa strain NDTH9845

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KCG38_RS18975
• Protein ID: WP_003160088.1
• Coordinates: 4098325..4098903 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KCG38_RS18970
• Protein ID: WP_003088112.1
• Coordinates: 4097683..4098237 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG38_RS18945 (KCG38_18945)	4093251..4093802	+	552	WP_003132721.1	XRE family transcriptional regulator	-
KCG38_RS18950 (KCG38_18950)	4093799..4094197	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
KCG38_RS18955 (KCG38_18955)	4094210..4094533	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
KCG38_RS18960 (KCG38_18960)	4094864..4095325	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
KCG38_RS18965 (KCG38_18965)	4095330..4097525	+	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
KCG38_RS18970 (KCG38_18970)	4097683..4098237	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
KCG38_RS18975 (KCG38_18975)	4098325..4098903	+	579	WP_003160088.1	HD domain-containing protein	Toxin
KCG38_RS18980 (KCG38_18980)	4099012..4100199	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KCG38_RS18985 (KCG38_18985)	4100189..4102360	-	2172	WP_009876279.1	type I secretion system permease/ATPase	-
KCG38_RS18990 (KCG38_18990)	4102350..4103627	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21816.65 Da
• Isoelectric Point: 5.1625

>T199558 WP_003160088.1 NZ_CP073080:4098325-4098903 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQCLAARRDA

Nucleotide

• Length: 579 bp

>T199558 NZ_CP097385:2674063-2674404 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT199558 WP_003088112.1 NZ_CP073080:4097683-4098237 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT199558 NZ_CP097385:2673725-2674042 [Klebsiella pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA