Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59304..59947 Replicon plasmid unnamed1
Accession NZ_CP073003
Organism Klebsiella pneumoniae strain FO15

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KBT91_RS27220 Protein ID WP_001044770.1
Coordinates 59304..59720 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KBT91_RS27225 Protein ID WP_001261282.1
Coordinates 59717..59947 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBT91_RS27200 (55407) 55407..55679 - 273 Protein_75 transposase -
KBT91_RS27210 (56661) 56661..57683 - 1023 WP_000361404.1 helicase UvrD -
KBT91_RS27215 (57668) 57668..59230 - 1563 WP_004206609.1 AAA family ATPase -
KBT91_RS27220 (59304) 59304..59720 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KBT91_RS27225 (59717) 59717..59947 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KBT91_RS27230 (59904) 59904..60365 + 462 WP_014343465.1 hypothetical protein -
KBT91_RS27235 (60526) 60526..61470 + 945 WP_011977810.1 hypothetical protein -
KBT91_RS27240 (61507) 61507..61899 + 393 WP_011977811.1 hypothetical protein -
KBT91_RS27245 (61957) 61957..62478 + 522 WP_013214008.1 hypothetical protein -
KBT91_RS27250 (62524) 62524..62727 + 204 WP_011977813.1 hypothetical protein -
KBT91_RS27255 (62757) 62757..63761 + 1005 WP_011977814.1 hypothetical protein -
KBT91_RS27260 (63945) 63945..64724 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid fosA3 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 / catA2 / blaCTX-M-65 - 1..144365 144365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T199480 WP_001044770.1 NZ_CP073003:c59720-59304 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T199480 NZ_CP073003:c59720-59304 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT199480 WP_001261282.1 NZ_CP073003:c59947-59717 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT199480 NZ_CP073003:c59947-59717 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References