Detailed information of TA system TA199375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3561548..3563136
• Replicon: chromosome
• Accession: NZ_CP072980
• Organism: Escherichia coli strain 2547

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A061YM43
• Locus tag: KBW66_RS17415
• Protein ID: WP_001125411.1
• Coordinates: 3561548..3562870 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A061YNW4
• Locus tag: KBW66_RS17420
• Protein ID: WP_001296076.1
• Coordinates: 3562870..3563136 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KBW66_RS17400 (3558225)	3558225..3558935	-	711	WP_001195176.1	fimbria/pilus periplasmic chaperone	-
KBW66_RS17405 (3559297)	3559297..3559860	-	564	WP_001296074.1	type 1 fimbrial major subunit FimA	-
KBW66_RS17410 (3560685)	3560685..3561382	+	698	WP_223293341.1	IS1-like element IS1B family transposase	-
KBW66_RS17415 (3561548)	3561548..3562870	-	1323	WP_001125411.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KBW66_RS17420 (3562870)	3562870..3563136	-	267	WP_001296076.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KBW66_RS17425 (3563444)	3563444..3564202	+	759	WP_001286537.1	trans-aconitate 2-methyltransferase	-
KBW66_RS17430 (3564206)	3564206..3565120	-	915	WP_001296077.1	bestrophin family protein	-
KBW66_RS17435 (3565317)	3565317..3566768	-	1452	WP_000854638.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49329.96 Da
• Isoelectric Point: 7.7906

>T199375 WP_001125411.1 NZ_CP072980:c3562870-3561548 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Nucleotide

• Length: 1323 bp

>T199375 NZ_CP097333:c53755-53327 [Mannheimia haemolytica]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9987.34 Da
• Isoelectric Point: 4.9632

>AT199375 WP_001296076.1 NZ_CP072980:c3563136-3562870 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT199375 NZ_CP097333:c54042-53755 [Mannheimia haemolytica]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Toxin

• AlphaFold DB: A0A061YM43

Antitoxin

• AlphaFold DB: A0A061YNW4