Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1243322..1244049 Replicon chromosome
Accession NZ_CP072980
Organism Escherichia coli strain 2547

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KBW66_RS05950 Protein ID WP_000550189.1
Coordinates 1243322..1243636 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KBW66_RS05955 Protein ID WP_000560269.1
Coordinates 1243633..1244049 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KBW66_RS05930 (1239479) 1239479..1240465 - 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -
KBW66_RS05935 (1240544) 1240544..1241236 - 693 WP_000942551.1 vancomycin high temperature exclusion protein -
KBW66_RS05940 (1241313) 1241313..1241816 - 504 WP_001295542.1 M48 family metallopeptidase -
KBW66_RS05945 (1241901) 1241901..1243037 + 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KBW66_RS05950 (1243322) 1243322..1243636 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KBW66_RS05955 (1243633) 1243633..1244049 + 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KBW66_RS05960 (1244094) 1244094..1246112 - 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KBW66_RS05965 (1246338) 1246338..1248689 - 2352 WP_000695432.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T199363 WP_000550189.1 NZ_CP072980:1243322-1243636 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T199363 NZ_CP097329:c2700288-2699785 [Pseudanabaena mucicola str. Chao 1806]
ATGAGTTTAGATAATTTGCGATCGCCTGAAAAGCTTAATTTATCTCACCAGATTAAGGGTTTTGACTCTGGCAATAGTCA
ATTAGATGACTGGTTAAAGAATAGAGCGATCAAAAATGAAATAGAAGGTGCTTCGCGTACCTATGTCCTGTGTAATGGTG
ATGTTGTGATTGGTTTCTATTGCCTCGCTAATGGCTCAGTGTTTCAATCTGTCGCTACGGGTAAGGTGCGGCGTAATATG
CCCGATCCGATTCCTGTAATGGTGATTGGAAGGTTAGCAATTGATCGCAGTTGGCAGGGCAAGGGGCTTGGTCGTGCCTT
GCTAAAGGACGCAATTTTAAGAACTCTACAAGCGTCTGAGATAGCGGGAATACGCGCTATCCTTGTGCATGCTATTTCTG
AGAATGCGAAGCTTTTTTATGAGAAATGTGGGTTTACGGTTTCGCCTATTGATGAGATGACTCTCATGATTAGGATTAAA
GATGCGATCACAGTATTCCAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT199363 WP_000560269.1 NZ_CP072980:1243633-1244049 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT199363 NZ_CP097329:c2700581-2700285 [Pseudanabaena mucicola str. Chao 1806]
ATGTCTACTTCAATAGATAACATCACATCTAGCCAAAATCGTGATGTCACGATTAACATCCGTGTGAAACAAGAGCAACG
GAATATCATTGATCGTGCTGCGGAAGTACAGGGTAAAACGCGCTCTGAGTTTATGCTTGAGTCAGCATATCAGAAAGCAC
AAGATGTTCTTTTAGATTGGGCTTTCTTTGATTTAGACCCATCGAAGTATCAGGAATTTACAGCATTATTGGATGGAGTT
CCTCAACCAAATCCAAAGTTACAGAAGTTACTAACAAATAAATCTCCGTGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References