Detailed information of TA system TA199064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1071401..1072005
• Replicon: chromosome
• Accession: NZ_CP072873
• Organism: Pseudomonas fluorescens strain JNU01

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J9321_RS04495
• Protein ID: WP_120447985.1
• Coordinates: 1071817..1072005 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J9321_RS04490
• Protein ID: WP_120447986.1
• Coordinates: 1071401..1071805 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9321_RS04465 (J9321_04465)	1066436..1067311	+	876	WP_120447988.1	urea transporter	-
J9321_RS04470 (J9321_04470)	1067343..1068167	+	825	WP_065901600.1	ion transporter	-
J9321_RS04475 (J9321_04475)	1068273..1069277	+	1005	WP_065901602.1	sulfate ABC transporter substrate-binding protein	-
J9321_RS04480 (J9321_04480)	1069405..1070019	-	615	WP_120447987.1	DUF5666 domain-containing protein	-
J9321_RS04485 (J9321_04485)	1070198..1071394	+	1197	WP_083221339.1	MFS transporter	-
J9321_RS04490 (J9321_04490)	1071401..1071805	-	405	WP_120447986.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J9321_RS04495 (J9321_04495)	1071817..1072005	-	189	WP_120447985.1	type II toxin-antitoxin system HicA family toxin	Toxin
J9321_RS04500 (J9321_04500)	1072232..1072888	+	657	WP_120447984.1	peroxiredoxin-like family protein	-
J9321_RS04505 (J9321_04505)	1072903..1073784	+	882	WP_120447983.1	LysR family transcriptional regulator	-
J9321_RS04510 (J9321_04510)	1073774..1075264	-	1491	WP_120447982.1	M10 family metallopeptidase C-terminal domain-containing protein	-
J9321_RS04515 (J9321_04515)	1075613..1076362	-	750	WP_106804708.1	phosphonate metabolism protein PhnP	-
J9321_RS04520 (J9321_04520)	1076353..1076925	-	573	WP_106804709.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7233.45 Da
• Isoelectric Point: 11.2000

>T199064 WP_120447985.1 NZ_CP072873:c1072005-1071817 [Pseudomonas fluorescens]
VQSRLLIKELQEAGWLLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T199064 NZ_CP097170:1259777-1260112 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTCAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTTGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14687.65 Da
• Isoelectric Point: 4.6000

>AT199064 WP_120447986.1 NZ_CP072873:c1071805-1071401 [Pseudomonas fluorescens]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGPIPMPTSVANHHAHEDYAGMGWGM
LELDVSPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT199064 NZ_CP097170:1259529-1259777 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCAGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGGCCTGGTGGATGGCAAATTAATTATTGAGCCAGTACGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAGCGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA