Detailed information of TA system TA198969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 475942..476579
• Replicon: chromosome
• Accession: NZ_CP072843
• Organism: Bacillus velezensis strain VY03

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: J9B93_RS02445
• Protein ID: WP_003156187.1
• Coordinates: 476229..476579 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: J9B93_RS02440
• Protein ID: WP_003156188.1
• Coordinates: 475942..476223 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9B93_RS02420 (J9B93_02420)	472307..472906	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
J9B93_RS02425 (J9B93_02425)	472999..473364	+	366	WP_014304402.1	holo-ACP synthase	-
J9B93_RS02430 (J9B93_02430)	473529..474536	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
J9B93_RS02435 (J9B93_02435)	474653..475822	+	1170	WP_003156189.1	alanine racemase	-
J9B93_RS02440 (J9B93_02440)	475942..476223	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
J9B93_RS02445 (J9B93_02445)	476229..476579	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
J9B93_RS02450 (J9B93_02450)	476697..477518	+	822	WP_014304404.1	STAS domain-containing protein	-
J9B93_RS02455 (J9B93_02455)	477523..477888	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
J9B93_RS02460 (J9B93_02460)	477891..478292	+	402	WP_003156178.1	anti-sigma regulatory factor	-
J9B93_RS02465 (J9B93_02465)	478304..479311	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
J9B93_RS02470 (J9B93_02470)	479375..479704	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
J9B93_RS02475 (J9B93_02475)	479701..480183	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
J9B93_RS02480 (J9B93_02480)	480149..480937	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
J9B93_RS02485 (J9B93_02485)	480937..481539	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T198969 WP_003156187.1 NZ_CP072843:476229-476579 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T198969 NZ_CP097076:c5144909-5144526 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT198969 WP_003156188.1 NZ_CP072843:475942-476223 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT198969 NZ_CP097076:c5145151-5144909 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5