Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 22705..23306 Replicon plasmid pPK5074-69kb
Accession NZ_CP072804
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name doc Uniprot ID A0A5U0JPI4
Locus tag J8617_RS24675 Protein ID WP_024166788.1
Coordinates 22926..23306 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A5T4YUW9
Locus tag J8617_RS24670 Protein ID WP_033545779.1
Coordinates 22705..22926 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS26410 17864..17977 - 114 Protein_19 hypothetical protein -
J8617_RS24625 (J8617_24610) 18114..18930 + 817 Protein_20 aminoglycoside O-phosphotransferase APH(3')-Ia -
J8617_RS24630 (J8617_24615) 19084..19995 - 912 Protein_21 C45 family autoproteolytic acyltransferase/hydolase -
J8617_RS24635 (J8617_24620) 20029..20367 - 339 Protein_22 Tn3 family transposase -
J8617_RS24640 (J8617_24625) 20420..21123 - 704 Protein_23 IS6-like element IS26 family transposase -
J8617_RS24645 (J8617_24630) 21114..21254 + 141 WP_107524640.1 DUF4752 family protein -
J8617_RS24650 (J8617_24635) 21251..21541 + 291 WP_048659782.1 hypothetical protein -
J8617_RS24655 (J8617_24640) 21611..21877 + 267 WP_000946114.1 hypothetical protein -
J8617_RS24660 (J8617_24645) 21880..22128 + 249 WP_001286530.1 DNA polymerase III subunit theta -
J8617_RS24665 (J8617_24650) 22240..22632 + 393 WP_000506718.1 S24 family peptidase -
J8617_RS24670 (J8617_24655) 22705..22926 + 222 WP_033545779.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
J8617_RS24675 (J8617_24660) 22926..23306 + 381 WP_024166788.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
J8617_RS24680 (J8617_24665) 23553..24053 - 501 Protein_31 Tn3 family transposase -
J8617_RS24685 (J8617_24670) 24119..24823 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
J8617_RS24690 (J8617_24675) 24877..25047 + 171 Protein_33 hypothetical protein -
J8617_RS24695 (J8617_24680) 25239..25718 + 480 WP_130560492.1 type 3 dihydrofolate reductase -
J8617_RS24700 (J8617_24685) 25739..25918 + 180 WP_000998543.1 type II toxin-antitoxin system CcdA family antitoxin -
J8617_RS24705 (J8617_24690) 26017..26436 - 420 WP_044866469.1 hypothetical protein -
J8617_RS24710 (J8617_24695) 26537..27234 + 698 WP_223429336.1 IS1 family transposase -
J8617_RS24715 (J8617_24700) 27435..28295 + 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(F) / aadA2 / qnrS1 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / blaTEM-1B - 1..69302 69302
- inside IScluster/Tn lnu(F) / aadA2 / qnrS1 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / blaTEM-1B - 3929..32003 28074


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13663.58 Da        Isoelectric Point: 5.6406

>T198915 WP_024166788.1 NZ_CP072804:22926-23306 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T198915 NZ_CP097056:2735976-2736119 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAACCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8147.13 Da        Isoelectric Point: 4.7708

>AT198915 WP_033545779.1 NZ_CP072804:22705-22926 [Escherichia coli]
MQSINFRTARGNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT198915 NZ_CP097056:c2736313-2736051 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5U0JPI4

Antitoxin

Source ID Structure
AlphaFold DB A0A5T4YUW9

References