Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1237079..1237710 Replicon chromosome
Accession NZ_CP072790
Organism Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag J8397_RS05930 Protein ID WP_003898728.1
Coordinates 1237079..1237390 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag J8397_RS05935 Protein ID WP_003405836.1
Coordinates 1237390..1237710 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8397_RS05910 (J8397_05805) 1232560..1233984 - 1425 WP_003405805.1 class II fumarate hydratase -
J8397_RS05915 (J8397_05810) 1234015..1235103 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
J8397_RS05920 (J8397_05815) 1235159..1235803 + 645 WP_003915494.1 DUF4245 domain-containing protein -
J8397_RS05925 (J8397_05820) 1235810..1236967 - 1158 WP_003898727.1 AI-2E family transporter -
J8397_RS05930 (J8397_05825) 1237079..1237390 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
J8397_RS05935 (J8397_05830) 1237390..1237710 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
J8397_RS05940 (J8397_05835) 1237720..1239256 + 1537 Protein_1169 carboxylesterase/lipase family protein -
J8397_RS05945 (J8397_05840) 1239263..1240375 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
J8397_RS05950 (J8397_05845) 1240385..1240642 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
J8397_RS05955 (J8397_05850) 1240632..1241879 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
J8397_RS05960 (J8397_05855) 1241876..1242514 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T198820 WP_003898728.1 NZ_CP072790:c1237390-1237079 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T198820 NZ_CP097020:c2571636-2571493 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT198820 WP_003405836.1 NZ_CP072790:c1237710-1237390 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT198820 NZ_CP097020:2571374-2571519 [Enterococcus faecalis]
TGTGCTATAATGAAAATGAAAAGAGAGATATGCTTCAACATACCTCTCTAGTGTAGAGCCGTTTGAGACGGTGACCGATT
ATATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References