Detailed information of TA system TA198812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 758022..758655
• Replicon: chromosome
• Accession: NZ_CP072790
• Organism: Mycobacterium tuberculosis strain I0003447-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J8397_RS03495
• Protein ID: WP_003912919.1
• Coordinates: 758022..758405 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: J8397_RS03500
• Protein ID: WP_003403368.1
• Coordinates: 758500..758655 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8397_RS03470 (J8397_03430)	753314..753850	+	537	WP_003403341.1	50S ribosomal protein L10	-
J8397_RS03475 (J8397_03435)	753887..754279	+	393	WP_070892741.1	50S ribosomal protein L7/L12	-
J8397_RS03480 (J8397_03440)	754272..754967	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
J8397_RS03485 (J8397_03445)	755038..756543	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
J8397_RS03490 (J8397_03450)	756624..757634	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
J8397_RS03495 (J8397_03455)	758022..758405	-	384	WP_003912919.1	ribonuclease VapC6	Toxin
J8397_RS03500 (J8397_03460)	758500..758655	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J8397_RS03505 (J8397_03465)	758731..759447	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
J8397_RS03510 (J8397_03470)	759723..760031	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
J8397_RS03515 (J8397_03475)	760018..760263	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
J8397_RS03520 (J8397_03480)	760373..760810	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
J8397_RS03525 (J8397_03485)	760807..761061	-	255	WP_003911263.1	antitoxin VapB7	-
J8397_RS03530 (J8397_03490)	761175..763538	+	2364	WP_003898547.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13989.76 Da
• Isoelectric Point: 6.0803

>T198812 WP_003912919.1 NZ_CP072790:c758405-758022 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTATARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T198812 NZ_CP097014:184865-185131 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT198812 WP_003403368.1 NZ_CP072790:c758655-758500 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT198812 NZ_CP097014:184602-184865 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8