Detailed information of TA system TA198322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2210994..2212582
• Replicon: chromosome
• Accession: NZ_CP072689
• Organism: Escherichia marmotae strain YF8

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J8I93_RS10435
• Protein ID: WP_061090838.1
• Coordinates: 2211260..2212582 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J8I93_RS10430
• Protein ID: WP_001516368.1
• Coordinates: 2210994..2211260 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8I93_RS10415 (2207671)	2207671..2208585	+	915	WP_001516366.1	bestrophin family protein	-
J8I93_RS10420 (2208589)	2208589..2209347	-	759	WP_061090837.1	trans-aconitate 2-methyltransferase	-
J8I93_RS10425 (2209774)	2209774..2210730	+	957	WP_000197006.1	hypothetical protein	-
J8I93_RS10430 (2210994)	2210994..2211260	+	267	WP_001516368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J8I93_RS10435 (2211260)	2211260..2212582	+	1323	WP_061090838.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
J8I93_RS10440 (2212940)	2212940..2213887	-	948	WP_282502953.1	hypothetical protein	-
J8I93_RS10445 (2214703)	2214703..2215794	-	1092	WP_061359330.1	cell envelope integrity TolA C-terminal domain-containing protein	-
J8I93_RS10450 (2216223)	2216223..2217080	+	858	WP_001516370.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2209774..2225792	16018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49555.22 Da
• Isoelectric Point: 8.8782

>T198322 WP_061090838.1 NZ_CP072689:2211260-2212582 [Escherichia marmotae]
MPKLVTWMNNQRVGELTKLTNGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPEDETVTRPIMVWEKLTEARLEEVLTAYKADIPLGMIREENNFRISVAGAQEKTAL
LRMGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKEFGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGSTDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGRKTEIDKIYPRHFLATAKALKFPEVQMHEILSDFARMIP
AALDNVKASLPADFPENVLTAIETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T198322 NZ_CP096845:c754041-753604 [Mycobacterium tuberculosis variant bovis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10034.37 Da
• Isoelectric Point: 4.5668

>AT198322 WP_001516368.1 NZ_CP072689:2210994-2211260 [Escherichia marmotae]
MMSFPMIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLNLSMTLCDTKNDSPES
TDQQDLEW

Nucleotide

• Length: 267 bp

>AT198322 NZ_CP096845:c753494-753249 [Mycobacterium tuberculosis variant bovis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA