Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 994324..994978 Replicon chromosome
Accession NZ_CP072687
Organism Escherichia coli strain YP3

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag J8I95_RS04945 Protein ID WP_000244781.1
Coordinates 994571..994978 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J8I95_RS04940 Protein ID WP_000354046.1
Coordinates 994324..994590 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I95_RS04920 (990412) 990412..991845 - 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -
J8I95_RS04925 (991890) 991890..992201 + 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
J8I95_RS04930 (992365) 992365..993024 + 660 WP_000250274.1 hemolysin III family protein -
J8I95_RS04935 (993101) 993101..994081 - 981 WP_000886078.1 tRNA-modifying protein YgfZ -
J8I95_RS04940 (994324) 994324..994590 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J8I95_RS04945 (994571) 994571..994978 + 408 WP_000244781.1 protein YgfX Toxin
J8I95_RS04950 (995018) 995018..995539 - 522 WP_001055874.1 flavodoxin FldB -
J8I95_RS04955 (995651) 995651..996547 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J8I95_RS04960 (996572) 996572..997282 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J8I95_RS04965 (997288) 997288..999021 + 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T198262 WP_000244781.1 NZ_CP072687:994571-994978 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T198262 NZ_CP096844:717546-717941 [Mycobacterium tuberculosis variant bovis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT198262 WP_000354046.1 NZ_CP072687:994324-994590 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT198262 NZ_CP096844:717292-717546 [Mycobacterium tuberculosis variant bovis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References