Detailed information of TA system TA198261

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 886728..887526
• Replicon: chromosome
• Accession: NZ_CP072687
• Organism: Escherichia coli strain YP3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZRI1
• Locus tag: J8I95_RS04350
• Protein ID: WP_000854738.1
• Coordinates: 886728..887105 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A067H947
• Locus tag: J8I95_RS04355
• Protein ID: WP_001285415.1
• Coordinates: 887152..887526 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8I95_RS04325 (882803)	882803..883786	-	984	WP_001331698.1	KpsF/GutQ family sugar-phosphate isomerase	-
J8I95_RS04330 (884588)	884588..884758	-	171	Protein_852	IS110 family transposase	-
J8I95_RS04335 (885101)	885101..885943	-	843	WP_020246044.1	DUF4942 domain-containing protein	-
J8I95_RS04340 (886028)	886028..886225	-	198	WP_000839268.1	DUF957 domain-containing protein	-
J8I95_RS04345 (886243)	886243..886731	-	489	WP_000761688.1	DUF5983 family protein	-
J8I95_RS04350 (886728)	886728..887105	-	378	WP_000854738.1	TA system toxin CbtA family protein	Toxin
J8I95_RS04355 (887152)	887152..887526	-	375	WP_001285415.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J8I95_RS04360 (887606)	887606..887827	-	222	WP_000692309.1	DUF987 domain-containing protein	-
J8I95_RS04365 (887890)	887890..888366	-	477	WP_021511973.1	RadC family protein	-
J8I95_RS04370 (888382)	888382..888861	-	480	WP_000844100.1	antirestriction protein	-
J8I95_RS04375 (888943)	888943..889761	-	819	WP_021573129.1	DUF932 domain-containing protein	-
J8I95_RS04380 (889851)	889851..890084	-	234	WP_001278287.1	DUF905 family protein	-
J8I95_RS04385 (890090)	890090..890767	-	678	WP_001097301.1	hypothetical protein	-
J8I95_RS04390 (890915)	890915..891595	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	884588..884692	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14089.12 Da
• Isoelectric Point: 8.2904

>T198261 WP_000854738.1 NZ_CP072687:c887105-886728 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMVRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T198261 NZ_CP096844:711887-712288 [Mycobacterium tuberculosis variant bovis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13663.32 Da
• Isoelectric Point: 5.8640

>AT198261 WP_001285415.1 NZ_CP072687:c887526-887152 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT198261 NZ_CP096844:711663-711890 [Mycobacterium tuberculosis variant bovis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: A7ZRI1

Antitoxin

• AlphaFold DB: A0A067H947