Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2633207..2633769 Replicon chromosome
Accession NZ_CP072686
Organism Escherichia coli strain FP4

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag J8I94_RS12820 Protein ID WP_000605675.1
Coordinates 2633207..2633485 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag J8I94_RS12825 Protein ID WP_000781370.1
Coordinates 2633485..2633769 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8I94_RS12795 (2628795) 2628795..2629226 - 432 WP_000152305.1 peroxiredoxin OsmC -
J8I94_RS12800 (2629571) 2629571..2629786 + 216 WP_000495766.1 biofilm-dependent modulation protein -
J8I94_RS12805 (2629888) 2629888..2630025 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
J8I94_RS12810 (2630181) 2630181..2631878 + 1698 WP_000433462.1 malate dehydrogenase -
J8I94_RS12815 (2632011) 2632011..2633021 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
J8I94_RS12820 (2633207) 2633207..2633485 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8I94_RS12825 (2633485) 2633485..2633769 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
J8I94_RS12830 (2633998) 2633998..2634651 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
J8I94_RS12835 (2634644) 2634644..2635528 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
J8I94_RS12840 (2635541) 2635541..2638588 - 3048 WP_077634457.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T198253 WP_000605675.1 NZ_CP072686:2633207-2633485 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T198253 NZ_CP096844:364342-364644 [Mycobacterium tuberculosis variant bovis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT198253 WP_000781370.1 NZ_CP072686:2633485-2633769 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT198253 NZ_CP096844:364118-364345 [Mycobacterium tuberculosis variant bovis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References